Restriction map for
pFN21AE5225
(Linear) (Six-base) MAPSORT of: KIEE5225.seq Check: 3109 from: 1 to: 750
KIEE5225.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AflIII A'CryG_T
Cuts at: 0 407 629 750
Size: 407 222 121
Fragments arranged by size:
407 222 121
AleI CACnn'nnGTG
Cuts at: 0 630 750
Size: 630 120
ApaI G_GGCC'C
Cuts at: 0 13 95 750
Size: 13 82 655
Fragments arranged by size:
655 82 13
AvaI C'yCGr_G
Cuts at: 0 67 88 124 719 750
Size: 67 21 36 595 31
Fragments arranged by size:
595 67 36 31 21
BamHI G'GATC_C
Cuts at: 0 75 708 750
Size: 75 633 42
Fragments arranged by size:
633 75 42
BanI G'GyrC_C
Cuts at: 0 21 43 750
Size: 21 22 707
Fragments arranged by size:
707 22 21
BanII G_rGCy'C
Cuts at: 0 13 95 241 750
Size: 13 82 146 509
Fragments arranged by size:
509 146 82 13
BbeI G_GCGC'C
Cuts at: 0 47 750
Size: 47 703
BbsI GAAGACnn'nnnn_
Cuts at: 0 537 750
Size: 537 213
BbvCI CC'TCA_GC
Cuts at: 0 493 750
Size: 493 257
BglI GCCn_nnn'nGGC
Cuts at: 0 99 120 750
Size: 99 21 630
Fragments arranged by size:
630 99 21
Bme1580I G_kGCm'C
Cuts at: 0 13 24 95 750
Size: 13 11 71 655
Fragments arranged by size:
655 71 13 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 750
Size: 354 396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 620 750
Size: 306 314 130
Fragments arranged by size:
314 306 130
Bpu10I CC'TnA_GC
Cuts at: 0 235 493 750
Size: 235 258 257
Fragments arranged by size:
258 257 235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 750
Size: 259 491
BsaI GGTCTCn'nnnn_
Cuts at: 0 325 750
Size: 325 425
BsaBI GATnn'nnATC
Cuts at: 0 371 750
Size: 371 379
BsaHI Gr'CG_yC
Cuts at: 0 44 280 750
Size: 44 236 470
Fragments arranged by size:
470 236 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 593 623 750
Size: 593 30 127
Fragments arranged by size:
593 127 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 750
Size: 196 554
BseYI C'CCAG_C
Cuts at: 0 94 243 257 750
Size: 94 149 14 493
Fragments arranged by size:
493 149 94 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 750
Size: 72 678
BsiHKAI G_wGCw'C
Cuts at: 0 241 750
Size: 241 509
Bsp1286I G_dGCh'C
Cuts at: 0 13 24 95 241 750
Size: 13 11 71 146 509
Fragments arranged by size:
509 146 71 13 11
BspHI T'CATG_A
Cuts at: 0 375 750
Size: 375 375
BsrBI CCG'CTC
Cuts at: 0 72 168 750
Size: 72 96 582
Fragments arranged by size:
582 96 72
BsrDI GCAATG_nn'
Cuts at: 0 63 750
Size: 63 687
BsrFI r'CCGG_y
Cuts at: 0 24 102 750
Size: 24 78 648
Fragments arranged by size:
648 78 24
BsrGI T'GTAC_A
Cuts at: 0 651 750
Size: 651 99
BssHII G'CGCG_C
Cuts at: 0 137 750
Size: 137 613
BstAPI GCAn_nnn'nTGC
Cuts at: 0 270 750
Size: 270 480
BstYI r'GATC_y
Cuts at: 0 75 708 750
Size: 75 633 42
Fragments arranged by size:
633 75 42
BtgI C'CryG_G
Cuts at: 0 37 82 118 146 750
Size: 37 45 36 28 604
Fragments arranged by size:
604 45 37 36 28
EaeI y'GGCC_r
Cuts at: 0 299 750
Size: 299 451
EarI CTCTTCn'nnn_
Cuts at: 0 719 750
Size: 719 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 43 68 121 750
Size: 43 25 53 629
Fragments arranged by size:
629 53 43 25
EcoICRI GAG'CTC
Cuts at: 0 239 750
Size: 239 511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 306 620 750
Size: 306 314 130
Fragments arranged by size:
314 306 130
EcoO109I rG'GnC_Cy
Cuts at: 0 9 10 750
Size: 9 1 740
Fragments arranged by size:
740 9 1
HaeII r_GCGC'y
Cuts at: 0 47 552 750
Size: 47 505 198
Fragments arranged by size:
505 198 47
KasI G'GCGC_C
Cuts at: 0 43 750
Size: 43 707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 750
Size: 426 324
MslI CAynn'nnrTG
Cuts at: 0 406 630 634 750
Size: 406 224 4 116
Fragments arranged by size:
406 224 116 4
MspA1I CmG'CkG
Cuts at: 0 39 84 120 148 261 750
Size: 39 45 36 28 113 489
Fragments arranged by size:
489 113 45 39 36 28
NaeI GCC'GGC
Cuts at: 0 104 750
Size: 104 646
NarI GG'CG_CC
Cuts at: 0 44 750
Size: 44 706
NgoMIV G'CCGG_C
Cuts at: 0 102 750
Size: 102 648
NspI r_CATG'y
Cuts at: 0 411 633 750
Size: 411 222 117
Fragments arranged by size:
411 222 117
PciI A'CATG_T
Cuts at: 0 407 629 750
Size: 407 222 121
Fragments arranged by size:
407 222 121
PflMI CCAn_nnn'nTGG
Cuts at: 0 445 750
Size: 445 305
PfoI T'CCnGG_A
Cuts at: 0 701 750
Size: 701 49
PspOMI G'GGCC_C
Cuts at: 0 9 91 750
Size: 9 82 659
Fragments arranged by size:
659 82 9
SacI G_AGCT'C
Cuts at: 0 241 750
Size: 241 509
SacII CC_GC'GG
Cuts at: 0 40 85 121 149 750
Size: 40 45 36 28 601
Fragments arranged by size:
601 45 40 36 28
SfcI C'TryA_G
Cuts at: 0 522 569 750
Size: 522 47 181
Fragments arranged by size:
522 181 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 99 750
Size: 99 651
SfoI GGC'GCC
Cuts at: 0 45 750
Size: 45 705
SgrAI Cr'CCGG_yG
Cuts at: 0 24 750
Size: 24 726
SmaI CCC'GGG
Cuts at: 0 90 750
Size: 90 660
SmlI C'TyrA_G
Cuts at: 0 274 750
Size: 274 476
SrfI GCCC'GGGC
Cuts at: 0 90 750
Size: 90 660
StyI C'CwwG_G
Cuts at: 0 317 486 750
Size: 317 169 264
Fragments arranged by size:
317 264 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 682 750
Size: 682 68
TatI w'GTAC_w
Cuts at: 0 651 750
Size: 651 99
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 687 750
Size: 687 63
XmaI C'CCGG_G
Cuts at: 0 88 750
Size: 88 662
Enzymes that do cut:
AflIII AleI ApaI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII KasI MmeI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PspOMI SacI SacII SfcI
SfiI SfoI SgrAI SmaI SmlI SrfI StyI TaqII
TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BsaAI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SalI SanDI
SapI SbfI ScaI SexAI SgfI SnaBI SpeI SphI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmnI
ZraI