Restriction map for
pFN21AE5224
(Linear) (Six-base) MAPSORT of: KIEE5224.seq Check: 618 from: 1 to: 582
KIEE5224.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 577 582
Size: 577 5
ApaLI G'TGCA_C
Cuts at: 0 400 582
Size: 400 182
ApoI r'AATT_y
Cuts at: 0 369 582
Size: 369 213
AvaI C'yCGr_G
Cuts at: 0 36 582
Size: 36 546
BanII G_rGCy'C
Cuts at: 0 473 582
Size: 473 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 503 582
Size: 503 79
BbvCI CC'TCA_GC
Cuts at: 0 76 582
Size: 76 506
BciVI GTATCCnnnnn_n'
Cuts at: 0 493 582
Size: 493 89
BglII A'GATC_T
Cuts at: 0 363 582
Size: 363 219
Bme1580I G_kGCm'C
Cuts at: 0 404 582
Size: 404 178
Bpu10I CC'TnA_GC
Cuts at: 0 76 582
Size: 76 506
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 582
Size: 379 203
BsaAI yAC'GTr
Cuts at: 0 123 405 582
Size: 123 282 177
Fragments arranged by size:
282 177 123
BsaWI w'CCGG_w
Cuts at: 0 50 582
Size: 50 532
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 553 582
Size: 451 102 29
Fragments arranged by size:
451 102 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 582
Size: 547 35
BsiHKAI G_wGCw'C
Cuts at: 0 37 404 473 582
Size: 37 367 69 109
Fragments arranged by size:
367 109 69 37
Bsp1286I G_dGCh'C
Cuts at: 0 37 404 473 582
Size: 37 367 69 109
Fragments arranged by size:
367 109 69 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 397 582
Size: 397 185
BsrGI T'GTAC_A
Cuts at: 0 191 582
Size: 191 391
BssSI C'ACGA_G
Cuts at: 0 433 582
Size: 433 149
BstYI r'GATC_y
Cuts at: 0 363 582
Size: 363 219
EaeI y'GGCC_r
Cuts at: 0 280 549 582
Size: 280 269 33
Fragments arranged by size:
280 269 33
EarI CTCTTCn'nnn_
Cuts at: 0 329 538 582
Size: 329 209 44
Fragments arranged by size:
329 209 44
EcoICRI GAG'CTC
Cuts at: 0 471 582
Size: 471 111
EcoRI G'AATT_C
Cuts at: 0 369 582
Size: 369 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 80 112 582
Size: 80 32 470
Fragments arranged by size:
470 80 32
HindIII A'AGCT_T
Cuts at: 0 127 582
Size: 127 455
MspA1I CmG'CkG
Cuts at: 0 15 582
Size: 15 567
NdeI CA'TA_TG
Cuts at: 0 180 248 582
Size: 180 68 334
Fragments arranged by size:
334 180 68
PasI CC'CwG_GG
Cuts at: 0 447 582
Size: 447 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 582
Size: 357 225
PfoI T'CCnGG_A
Cuts at: 0 132 422 582
Size: 132 290 160
Fragments arranged by size:
290 160 132
PmlI CAC'GTG
Cuts at: 0 123 405 582
Size: 123 282 177
Fragments arranged by size:
282 177 123
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 582
Size: 102 32 448
Fragments arranged by size:
448 102 32
PspXI vC'TCGA_Gb
Cuts at: 0 36 582
Size: 36 546
PstI C_TGCA'G
Cuts at: 0 530 582
Size: 530 52
SacI G_AGCT'C
Cuts at: 0 473 582
Size: 473 109
SexAI A'CCwGG_T
Cuts at: 0 350 582
Size: 350 232
SfcI C'TryA_G
Cuts at: 0 526 582
Size: 526 56
SmlI C'TyrA_G
Cuts at: 0 36 582
Size: 36 546
TatI w'GTAC_w
Cuts at: 0 191 582
Size: 191 391
XhoI C'TCGA_G
Cuts at: 0 36 582
Size: 36 546
Enzymes that do cut:
AlfI ApaLI ApoI AvaI BanII BbsI BbvCI BciVI
BglII Bme1580I Bpu10I BsaI BsaAI BsaWI BseRI BsgI
BsiHKAI Bsp1286I BspMI BsrGI BssSI BstYI EaeI EarI
EcoICRI EcoRI Hin4I HindIII MspA1I NdeI PasI PflMI
PfoI PmlI PpiI PspXI PstI SacI SexAI SfcI
SmlI TatI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
BpmI BpuEI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PpuMI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XcmI XmaI XmnI
ZraI