Restriction map for
pFN21AE5223
(Linear) (Six-base) MAPSORT of: KIEE5223.seq Check: 7187 from: 1 to: 747
KIEE5223.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 162 747
Size: 162 585
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 448 495 747
Size: 25 423 47 252
Fragments arranged by size:
423 252 47 25
AleI CACnn'nnGTG
Cuts at: 0 148 602 747
Size: 148 454 145
Fragments arranged by size:
454 148 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 747
Size: 211 536
AlwNI CAG_nnn'CTG
Cuts at: 0 543 747
Size: 543 204
ApaLI G'TGCA_C
Cuts at: 0 268 747
Size: 268 479
AvaI C'yCGr_G
Cuts at: 0 653 747
Size: 653 94
BanII G_rGCy'C
Cuts at: 0 257 317 346 407 458 747
Size: 257 60 29 61 51 289
Fragments arranged by size:
289 257 61 60 51 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 57 501 747
Size: 57 444 246
Fragments arranged by size:
444 246 57
BlpI GC'TnA_GC
Cuts at: 0 286 747
Size: 286 461
Bme1580I G_kGCm'C
Cuts at: 0 272 747
Size: 272 475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 49 79 601 655 726 747
Size: 43 6 30 522 54 71 21
Fragments arranged by size:
522 71 54 43 30 21 6
BsaWI w'CCGG_w
Cuts at: 0 275 479 747
Size: 275 204 268
Fragments arranged by size:
275 268 204
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 577 747
Size: 415 162 170
Fragments arranged by size:
415 170 162
BseYI C'CCAG_C
Cuts at: 0 339 515 747
Size: 339 176 232
Fragments arranged by size:
339 232 176
BsiHKAI G_wGCw'C
Cuts at: 0 272 317 407 458 747
Size: 272 45 90 51 289
Fragments arranged by size:
289 272 90 51 45
BsmBI CGTCTCn'nnnn_
Cuts at: 0 210 378 747
Size: 210 168 369
Fragments arranged by size:
369 210 168
Bsp1286I G_dGCh'C
Cuts at: 0 257 272 317 346 407 458 747
Size: 257 15 45 29 61 51 289
Fragments arranged by size:
289 257 61 51 45 29 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 162 747
Size: 162 585
BsrBI CCG'CTC
Cuts at: 0 285 660 747
Size: 285 375 87
Fragments arranged by size:
375 285 87
BsrFI r'CCGG_y
Cuts at: 0 35 747
Size: 35 712
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 747
Size: 113 634
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 747
Size: 57 690
Bsu36I CC'TnA_GG
Cuts at: 0 232 747
Size: 232 515
BtgI C'CryG_G
Cuts at: 0 603 734 747
Size: 603 131 13
Fragments arranged by size:
603 131 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 444 747
Size: 444 303
DraIII CAC_nnn'GTG
Cuts at: 0 555 747
Size: 555 192
EaeI y'GGCC_r
Cuts at: 0 93 747
Size: 93 654
EarI CTCTTCn'nnn_
Cuts at: 0 421 747
Size: 421 326
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 514 747
Size: 514 233
EcoICRI GAG'CTC
Cuts at: 0 315 405 456 747
Size: 315 90 51 291
Fragments arranged by size:
315 291 90 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 43 49 79 448 495 601 655
Size: 25 18 6 30 369 47 106 54
Cuts at: 655 726 747
Size: 71 21
Fragments arranged by size:
369 106 71 54 47 30 25 21
18 6
EcoO109I rG'GnC_Cy
Cuts at: 0 246 747
Size: 246 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 579 611 747
Size: 579 32 136
Fragments arranged by size:
579 136 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 747
Size: 482 265
MslI CAynn'nnrTG
Cuts at: 0 148 602 747
Size: 148 454 145
Fragments arranged by size:
454 148 145
MspA1I CmG'CkG
Cuts at: 0 515 575 633 747
Size: 515 60 58 114
Fragments arranged by size:
515 114 60 58
NcoI C'CATG_G
Cuts at: 0 734 747
Size: 734 13
NspI r_CATG'y
Cuts at: 0 630 747
Size: 630 117
PasI CC'CwG_GG
Cuts at: 0 241 645 747
Size: 241 404 102
Fragments arranged by size:
404 241 102
PflMI CCAn_nnn'nTGG
Cuts at: 0 167 555 735 747
Size: 167 388 180 12
Fragments arranged by size:
388 180 167 12
PfoI T'CCnGG_A
Cuts at: 0 536 747
Size: 536 211
PpuMI rG'GwC_Cy
Cuts at: 0 246 747
Size: 246 501
PstI C_TGCA'G
Cuts at: 0 388 747
Size: 388 359
PvuII CAG'CTG
Cuts at: 0 515 633 747
Size: 515 118 114
Fragments arranged by size:
515 118 114
SacI G_AGCT'C
Cuts at: 0 317 407 458 747
Size: 317 90 51 289
Fragments arranged by size:
317 289 90 51
SanDI GG'GwC_CC
Cuts at: 0 246 747
Size: 246 501
SfcI C'TryA_G
Cuts at: 0 85 384 747
Size: 85 299 363
Fragments arranged by size:
363 299 85
SmaI CCC'GGG
Cuts at: 0 655 747
Size: 655 92
StyI C'CwwG_G
Cuts at: 0 146 257 734 747
Size: 146 111 477 13
Fragments arranged by size:
477 146 111 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 207 507 747
Size: 207 300 240
Fragments arranged by size:
300 240 207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 747
Size: 207 540
XmaI C'CCGG_G
Cuts at: 0 653 747
Size: 653 94
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaLI AvaI BanII
BbsI BlpI Bme1580I BpmI BsaWI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI Bsu36I
BtgI BtgZI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I Hin4I MmeI MslI MspA1I NcoI NspI PasI
PflMI PfoI PpuMI PstI PvuII SacI SanDI SfcI
SmaI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstYI BstZ17I BtsI ClaI CspCI DraI
DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmnI ZraI