Restriction map for
pFN21AE5222
(Linear) (Six-base) MAPSORT of: KIEE5222.seq Check: 2818 from: 1 to: 768
KIEE5222.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 768
Size: 85 683
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 235 267 768
Size: 235 32 501
Fragments arranged by size:
501 235 32
AlwNI CAG_nnn'CTG
Cuts at: 0 60 63 681 768
Size: 60 3 618 87
Fragments arranged by size:
618 87 60 3
AvaI C'yCGr_G
Cuts at: 0 368 768
Size: 368 400
BanII G_rGCy'C
Cuts at: 0 11 620 768
Size: 11 609 148
Fragments arranged by size:
609 148 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 73 315 727 768
Size: 73 242 412 41
Fragments arranged by size:
412 242 73 41
BbvCI CC'TCA_GC
Cuts at: 0 661 768
Size: 661 107
BmgBI CAC'GTC
Cuts at: 0 331 768
Size: 331 437
BmrI ACTGGGnnnn_n'
Cuts at: 0 5 768
Size: 5 763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 709 768
Size: 607 102 59
Fragments arranged by size:
607 102 59
Bpu10I CC'TnA_GC
Cuts at: 0 661 768
Size: 661 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 768
Size: 43 725
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 120 150 320 350 768
Size: 120 30 170 30 418
Fragments arranged by size:
418 170 120 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 141 328 550 553 768
Size: 34 107 187 222 3 215
Fragments arranged by size:
222 215 187 107 34 3
BseYI C'CCAG_C
Cuts at: 0 53 401 512 674 768
Size: 53 348 111 162 94
Fragments arranged by size:
348 162 111 94 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 768
Size: 550 218
BsiEI CG_ry'CG
Cuts at: 0 713 768
Size: 713 55
BsiHKAI G_wGCw'C
Cuts at: 0 387 620 768
Size: 387 233 148
Fragments arranged by size:
387 233 148
Bsp1286I G_dGCh'C
Cuts at: 0 11 387 620 768
Size: 11 376 233 148
Fragments arranged by size:
376 233 148 11
BsrFI r'CCGG_y
Cuts at: 0 344 712 768
Size: 344 368 56
Fragments arranged by size:
368 344 56
BstYI r'GATC_y
Cuts at: 0 183 768
Size: 183 585
Bsu36I CC'TnA_GG
Cuts at: 0 445 768
Size: 445 323
EaeI y'GGCC_r
Cuts at: 0 212 710 768
Size: 212 498 58
Fragments arranged by size:
498 212 58
EagI C'GGCC_G
Cuts at: 0 710 768
Size: 710 58
EarI CTCTTCn'nnn_
Cuts at: 0 505 768
Size: 505 263
EcoICRI GAG'CTC
Cuts at: 0 618 768
Size: 618 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 607 709 768
Size: 85 522 102 59
Fragments arranged by size:
522 102 85 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 320 352 768
Size: 320 32 416
Fragments arranged by size:
416 320 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 768
Size: 351 417
MscI TGG'CCA
Cuts at: 0 214 768
Size: 214 554
MslI CAynn'nnrTG
Cuts at: 0 463 768
Size: 463 305
MspA1I CmG'CkG
Cuts at: 0 60 164 378 564 585 678 733 768
Size: 60 104 214 186 21 93 55 35
Fragments arranged by size:
214 186 104 93 60 55 35 21
NaeI GCC'GGC
Cuts at: 0 714 768
Size: 714 54
NgoMIV G'CCGG_C
Cuts at: 0 712 768
Size: 712 56
NspI r_CATG'y
Cuts at: 0 276 462 768
Size: 276 186 306
Fragments arranged by size:
306 276 186
PfoI T'CCnGG_A
Cuts at: 0 632 768
Size: 632 136
PstI C_TGCA'G
Cuts at: 0 242 353 398 563 768
Size: 242 111 45 165 205
Fragments arranged by size:
242 205 165 111 45
PvuII CAG'CTG
Cuts at: 0 60 378 564 585 678 733 768
Size: 60 318 186 21 93 55 35
Fragments arranged by size:
318 186 93 60 55 35 21
SacI G_AGCT'C
Cuts at: 0 620 768
Size: 620 148
SbfI CC_TGCA'GG
Cuts at: 0 398 768
Size: 398 370
SfcI C'TryA_G
Cuts at: 0 132 238 349 394 559 750 768
Size: 132 106 111 45 165 191 18
Fragments arranged by size:
191 165 132 111 106 45 18
SspI AAT'ATT
Cuts at: 0 112 768
Size: 112 656
TatI w'GTAC_w
Cuts at: 0 176 768
Size: 176 592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 471 768
Size: 222 249 297
Fragments arranged by size:
297 249 222
Enzymes that do cut:
AcuI AloI AlwNI AvaI BanII BbsI BbvCI BmgBI
BmrI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BsrFI BstYI Bsu36I EaeI EagI
EarI EcoICRI Eco57MI Hin4I MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PfoI PstI PvuII SacI SbfI
SfcI SspI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmtI
BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EciI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI