Restriction map for
pFN21AE5221
(Linear) (Six-base) MAPSORT of: KIEE5221.seq Check: 6657 from: 1 to: 777
KIEE5221.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
Acc65I G'GTAC_C
Cuts at: 0 716 777
Size: 716 61
AlwNI CAG_nnn'CTG
Cuts at: 0 180 777
Size: 180 597
ApoI r'AATT_y
Cuts at: 0 727 777
Size: 727 50
BanI G'GyrC_C
Cuts at: 0 716 777
Size: 716 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 777
Size: 72 705
BciVI GTATCCnnnnn_n'
Cuts at: 0 538 749 777
Size: 538 211 28
Fragments arranged by size:
538 211 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 441 777
Size: 441 336
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 777
Size: 201 576
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 777
Size: 423 354
BsaAI yAC'GTr
Cuts at: 0 579 777
Size: 579 198
BsaBI GATnn'nnATC
Cuts at: 0 493 777
Size: 493 284
BsaWI w'CCGG_w
Cuts at: 0 722 777
Size: 722 55
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 335 777
Size: 40 295 442
Fragments arranged by size:
442 295 40
BseYI C'CCAG_C
Cuts at: 0 130 777
Size: 130 647
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 434 777
Size: 193 241 343
Fragments arranged by size:
343 241 193
BsiEI CG_ry'CG
Cuts at: 0 117 777
Size: 117 660
BsmBI CGTCTCn'nnnn_
Cuts at: 0 238 777
Size: 238 539
BspEI T'CCGG_A
Cuts at: 0 722 777
Size: 722 55
BspHI T'CATG_A
Cuts at: 0 708 777
Size: 708 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 777
Size: 62 715
BsrBI CCG'CTC
Cuts at: 0 201 777
Size: 201 576
BsrFI r'CCGG_y
Cuts at: 0 150 777
Size: 150 627
BsrGI T'GTAC_A
Cuts at: 0 349 777
Size: 349 428
BssHII G'CGCG_C
Cuts at: 0 242 777
Size: 242 535
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 421 777
Size: 180 241 356
Fragments arranged by size:
356 241 180
BstYI r'GATC_y
Cuts at: 0 441 777
Size: 441 336
Bsu36I CC'TnA_GG
Cuts at: 0 514 777
Size: 514 263
BtgI C'CryG_G
Cuts at: 0 65 777
Size: 65 712
BtsI GCAGTG_nn'
Cuts at: 0 64 422 777
Size: 64 358 355
Fragments arranged by size:
358 355 64
EaeI y'GGCC_r
Cuts at: 0 38 114 263 632 777
Size: 38 76 149 369 145
Fragments arranged by size:
369 149 145 76 38
EagI C'GGCC_G
Cuts at: 0 114 777
Size: 114 663
EarI CTCTTCn'nnn_
Cuts at: 0 728 777
Size: 728 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 213 777
Size: 37 176 564
Fragments arranged by size:
564 176 37
HaeII r_GCGC'y
Cuts at: 0 33 777
Size: 33 744
HincII GTy'rAC
Cuts at: 0 476 777
Size: 476 301
HpaI GTT'AAC
Cuts at: 0 476 777
Size: 476 301
KpnI G_GTAC'C
Cuts at: 0 720 777
Size: 720 57
MscI TGG'CCA
Cuts at: 0 265 634 777
Size: 265 369 143
Fragments arranged by size:
369 265 143
MspA1I CmG'CkG
Cuts at: 0 638 777
Size: 638 139
NaeI GCC'GGC
Cuts at: 0 152 777
Size: 152 625
NgoMIV G'CCGG_C
Cuts at: 0 150 777
Size: 150 627
NspI r_CATG'y
Cuts at: 0 486 777
Size: 486 291
PsiI TTA'TAA
Cuts at: 0 760 777
Size: 760 17
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 94 126 730 762 777
Size: 94 32 604 32 15
Fragments arranged by size:
604 94 32 32 15
PstI C_TGCA'G
Cuts at: 0 59 588 777
Size: 59 529 189
Fragments arranged by size:
529 189 59
PvuII CAG'CTG
Cuts at: 0 638 777
Size: 638 139
SfcI C'TryA_G
Cuts at: 0 55 584 777
Size: 55 529 193
Fragments arranged by size:
529 193 55
SmlI C'TyrA_G
Cuts at: 0 402 777
Size: 402 375
SnaBI TAC'GTA
Cuts at: 0 579 777
Size: 579 198
StyI C'CwwG_G
Cuts at: 0 556 648 777
Size: 556 92 129
Fragments arranged by size:
556 129 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 777
Size: 156 621
TatI w'GTAC_w
Cuts at: 0 349 777
Size: 349 428
Enzymes that do cut:
Acc65I AlwNI ApoI BanI BbsI BciVI BmrI BplI
BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiEI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstYI Bsu36I BtgI BtsI EaeI EagI EarI
EciI HaeII HincII HpaI KpnI MscI MspA1I NaeI
NgoMIV NspI PsiI PsrI PstI PvuII SfcI SmlI
SnaBI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BpmI Bpu10I BsaI BsaHI BsaXI BsiHKAI
BsiWI BsmI Bsp1286I BsrDI BssSI BstBI BstEII BstXI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII KasI MfeI MluI MmeI MslI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI