(Linear) (Six-base) MAPSORT of: KIEE5220.seq  Check: 5803  from: 1  to: 678



KIEE5220.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:51  ..

AccI GT'mk_AC

Cuts at:      0     126     242     678 
   Size:        126     116     436

  Fragments arranged by size: 

                 436     126     116
 
ApoI r'AATT_y

Cuts at:      0     132     211     375     609     669     678 
   Size:        132      79     164     234      60       9

  Fragments arranged by size: 

                 234     164     132      79      60       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     147     678 
   Size:        147     531
 
BlpI GC'TnA_GC

Cuts at:      0      77     678 
   Size:         77     601
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      34     678 
   Size:         34     644
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     678 
   Size:        185     493
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     678 
   Size:         31     647
 
BsaAI yAC'GTr

Cuts at:      0     493     678 
   Size:        493     185
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     188     678 
   Size:        188     490
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     678 
   Size:         10     668
 
BsrDI GCAATG_nn'

Cuts at:      0     592     678 
   Size:        592      86
 
BsrGI T'GTAC_A

Cuts at:      0     416     678 
   Size:        416     262
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     396     678 
   Size:        396     282
 
BstYI r'GATC_y

Cuts at:      0     645     678 
   Size:        645      33
 
BtsI GCAGTG_nn'

Cuts at:      0      71     678 
   Size:         71     607
 
DraI TTT'AAA

Cuts at:      0     674     678 
   Size:        674       4
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     185     678 
   Size:        185     493
 
EcoRI G'AATT_C

Cuts at:      0     132     678 
   Size:        132     546
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     678 
   Size:        183     495
 
MslI CAynn'nnrTG

Cuts at:      0     236     495     678 
   Size:        236     259     183

  Fragments arranged by size: 

                 259     236     183
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     253     678 
   Size:        253     425
 
PsiI TTA'TAA

Cuts at:      0      62     217     678 
   Size:         62     155     461

  Fragments arranged by size: 

                 461     155      62
 
SfcI C'TryA_G

Cuts at:      0     127     334     582     649     678 
   Size:        127     207     248      67      29

  Fragments arranged by size: 

                 248     207     127      67      29
 
SmlI C'TyrA_G

Cuts at:      0      46     678 
   Size:         46     632
 
TatI w'GTAC_w

Cuts at:      0     185     416     678 
   Size:        185     231     262

  Fragments arranged by size: 

                 262     231     185
 
Tth111I GACn'n_nGTC

Cuts at:      0     546     678 
   Size:        546     132
 

 Enzymes that do cut:

     AccI     ApoI     BbsI     BlpI     BplI     BpmI    BpuEI    BsaAI
    BseRI     BsgI    BsrDI    BsrGI    BstXI    BstYI     BtsI     DraI
  Eco57MI    EcoRI     MmeI     MslI    PflMI     PsiI     SfcI     SmlI
     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI