(Linear) (Six-base) MAPSORT of: KIEE5219.seq  Check: 3602  from: 1  to: 618



KIEE5219.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:51  ..

AccI GT'mk_AC

Cuts at:      0     608     618 
   Size:        608      10
 
ApoI r'AATT_y

Cuts at:      0     285     458     575     618 
   Size:        285     173     117      43

  Fragments arranged by size: 

                 285     173     117      43
 
BanI G'GyrC_C

Cuts at:      0      14      71     618 
   Size:         14      57     547

  Fragments arranged by size: 

                 547      57      14
 
BanII G_rGCy'C

Cuts at:      0     163     341     618 
   Size:        163     178     277

  Fragments arranged by size: 

                 277     178     163
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     442     476     588     618 
   Size:        442      34     112      30

  Fragments arranged by size: 

                 442     112      34      30
 
BclI T'GATC_A

Cuts at:      0     470     618 
   Size:        470     148
 
BglI GCCn_nnn'nGGC

Cuts at:      0     345     618 
   Size:        345     273
 
Bme1580I G_kGCm'C

Cuts at:      0      76     618 
   Size:         76     542
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     618 
   Size:        195     423
 
Bpu10I CC'TnA_GC

Cuts at:      0      31     618 
   Size:         31     587
 
BsaBI GATnn'nnATC

Cuts at:      0     358     618 
   Size:        358     260
 
BsaWI w'CCGG_w

Cuts at:      0     431     618 
   Size:        431     187
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     240     270     618 
   Size:        240      30     348

  Fragments arranged by size: 

                 348     240      30
 
BseYI C'CCAG_C

Cuts at:      0      23     618 
   Size:         23     595
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     618 
   Size:         73     545
 
Bsp1286I G_dGCh'C

Cuts at:      0      76     163     341     618 
   Size:         76      87     178     277

  Fragments arranged by size: 

                 277     178      87      76
 
BspEI T'CCGG_A

Cuts at:      0     431     618 
   Size:        431     187
 
DraIII CAC_nnn'GTG

Cuts at:      0      14     618 
   Size:         14     604
 
EaeI y'GGCC_r

Cuts at:      0       5     618 
   Size:          5     613
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     618 
   Size:        195     423
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     180     618 
   Size:         27     153     438

  Fragments arranged by size: 

                 438     153      27
 
EcoRI G'AATT_C

Cuts at:      0     285     575     618 
   Size:        285     290      43

  Fragments arranged by size: 

                 290     285      43
 
HaeII r_GCGC'y

Cuts at:      0      88     618 
   Size:         88     530
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29      61     240     272     383     415     455     487 
   Size:         29      32     179      32     111      32      40      32

Cuts at:    487     618 
   Size:        131

  Fragments arranged by size: 

                 179     131     111      40      32      32      32      32
                  29
 
HincII GTy'rAC

Cuts at:      0     609     618 
   Size:        609       9
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     618 
   Size:        226     392
 
PpuMI rG'GwC_Cy

Cuts at:      0     180     618 
   Size:        180     438
 
PstI C_TGCA'G

Cuts at:      0     455     618 
   Size:        455     163
 
SalI G'TCGA_C

Cuts at:      0     607     618 
   Size:        607      11
 
SanDI GG'GwC_CC

Cuts at:      0     180     618 
   Size:        180     438
 
SexAI A'CCwGG_T

Cuts at:      0      10     618 
   Size:         10     608
 
SfcI C'TryA_G

Cuts at:      0     126     451     618 
   Size:        126     325     167

  Fragments arranged by size: 

                 325     167     126
 
StyI C'CwwG_G

Cuts at:      0     290     618 
   Size:        290     328
 
XbaI T'CTAG_A

Cuts at:      0     219     618 
   Size:        219     399
 
XmnI GAAnn'nnTTC

Cuts at:      0     579     618 
   Size:        579      39
 

 Enzymes that do cut:

     AccI     ApoI     BanI    BanII     BcgI     BclI     BglI Bme1580I
     BpmI   Bpu10I    BsaBI    BsaWI    BsaXI    BseYI     BsgI Bsp1286I
    BspEI   DraIII     EaeI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I
   HincII     MmeI    PpuMI     PstI     SalI    SanDI    SexAI     SfcI
     StyI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI    BciVI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaHI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI