Restriction map for
pFN21AE5218
(Linear) (Six-base) MAPSORT of: KIEE5218.seq Check: 9830 from: 1 to: 570
KIEE5218.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
Acc65I G'GTAC_C
Cuts at: 0 473 570
Size: 473 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 507 570
Size: 65 442 63
Fragments arranged by size:
442 65 63
AlwNI CAG_nnn'CTG
Cuts at: 0 381 570
Size: 381 189
AvaI C'yCGr_G
Cuts at: 0 266 570
Size: 266 304
BanI G'GyrC_C
Cuts at: 0 473 570
Size: 473 97
BciVI GTATCCnnnnn_n'
Cuts at: 0 505 570
Size: 505 65
BclI T'GATC_A
Cuts at: 0 185 570
Size: 185 385
BlpI GC'TnA_GC
Cuts at: 0 382 570
Size: 382 188
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 407 570
Size: 407 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 230 570
Size: 218 12 340
Fragments arranged by size:
340 218 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 570
Size: 241 329
BsaWI w'CCGG_w
Cuts at: 0 175 570
Size: 175 395
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 203 233 570
Size: 203 30 337
Fragments arranged by size:
337 203 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 119 570
Size: 119 451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 570
Size: 397 173
BsmI GAATG_Cn'
Cuts at: 0 299 570
Size: 299 271
BspHI T'CATG_A
Cuts at: 0 458 570
Size: 458 112
BsrDI GCAATG_nn'
Cuts at: 0 245 482 561 570
Size: 245 237 79 9
Fragments arranged by size:
245 237 79 9
BsrFI r'CCGG_y
Cuts at: 0 502 570
Size: 502 68
BstAPI GCAn_nnn'nTGC
Cuts at: 0 378 408 570
Size: 378 30 162
Fragments arranged by size:
378 162 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 570
Size: 348 222
BtgI C'CryG_G
Cuts at: 0 96 570
Size: 96 474
BtsI GCAGTG_nn'
Cuts at: 0 230 545 570
Size: 230 315 25
Fragments arranged by size:
315 230 25
EarI CTCTTCn'nnn_
Cuts at: 0 526 570
Size: 526 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 218 230 507 570
Size: 65 153 12 277 63
Fragments arranged by size:
277 153 65 63 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 323 570
Size: 323 247
EcoRV GAT'ATC
Cuts at: 0 552 570
Size: 552 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 503 535 570
Size: 503 32 35
Fragments arranged by size:
503 35 32
KpnI G_GTAC'C
Cuts at: 0 477 570
Size: 477 93
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 570
Size: 65 505
MslI CAynn'nnrTG
Cuts at: 0 95 121 346 424 457 570
Size: 95 26 225 78 33 113
Fragments arranged by size:
225 113 95 78 33 26
MspA1I CmG'CkG
Cuts at: 0 196 527 570
Size: 196 331 43
Fragments arranged by size:
331 196 43
NcoI C'CATG_G
Cuts at: 0 96 570
Size: 96 474
NdeI CA'TA_TG
Cuts at: 0 17 570
Size: 17 553
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 570
Size: 18 552
PvuII CAG'CTG
Cuts at: 0 196 527 570
Size: 196 331 43
Fragments arranged by size:
331 196 43
SmlI C'TyrA_G
Cuts at: 0 256 570
Size: 256 314
StyI C'CwwG_G
Cuts at: 0 96 570
Size: 96 474
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 86 570
Size: 86 484
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 203 235 570
Size: 203 32 335
Fragments arranged by size:
335 203 32
Enzymes that do cut:
Acc65I AcuI AlwNI AvaI BanI BciVI BclI BlpI
BplI BpmI BpuEI BsaWI BsaXI BseRI BsgI BsmI
BspHI BsrDI BsrFI BstAPI BstXI BtgI BtsI EarI
Eco57MI EcoNI EcoRV Hin4I KpnI MmeI MslI MspA1I
NcoI NdeI PflMI PvuII SmlI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BfrBI BglI BglII Bme1580I BmgBI BmrI
BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BseYI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI