Restriction map for
pFN21AE5217
(Linear) (Six-base) MAPSORT of: KIEE5217.seq Check: 6134 from: 1 to: 612
KIEE5217.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 311 612
Size: 311 301
AlwNI CAG_nnn'CTG
Cuts at: 0 333 612
Size: 333 279
ApaLI G'TGCA_C
Cuts at: 0 462 612
Size: 462 150
AscI GG'CGCG_CC
Cuts at: 0 387 612
Size: 387 225
AvaI C'yCGr_G
Cuts at: 0 362 612
Size: 362 250
BanII G_rGCy'C
Cuts at: 0 530 550 612
Size: 530 20 62
Fragments arranged by size:
530 62 20
Bme1580I G_kGCm'C
Cuts at: 0 466 612
Size: 466 146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 612
Size: 607 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 612
Size: 31 581
BsaWI w'CCGG_w
Cuts at: 0 488 612
Size: 488 124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 540 612
Size: 540 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 176 612
Size: 103 73 436
Fragments arranged by size:
436 103 73
BsiEI CG_ry'CG
Cuts at: 0 240 612
Size: 240 372
BsiHKAI G_wGCw'C
Cuts at: 0 73 466 530 612
Size: 73 393 64 82
Fragments arranged by size:
393 82 73 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 396 612
Size: 396 216
Bsp1286I G_dGCh'C
Cuts at: 0 73 466 530 550 612
Size: 73 393 64 20 62
Fragments arranged by size:
393 73 64 62 20
BspEI T'CCGG_A
Cuts at: 0 488 612
Size: 488 124
BspHI T'CATG_A
Cuts at: 0 260 612
Size: 260 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 311 318 612
Size: 152 159 7 294
Fragments arranged by size:
294 159 152 7
BsrDI GCAATG_nn'
Cuts at: 0 113 503 612
Size: 113 390 109
Fragments arranged by size:
390 113 109
BssHII G'CGCG_C
Cuts at: 0 387 612
Size: 387 225
BssSI C'ACGA_G
Cuts at: 0 473 612
Size: 473 139
BtsI GCAGTG_nn'
Cuts at: 0 125 612
Size: 125 487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 363 398 612
Size: 363 35 214
Fragments arranged by size:
363 214 35
EaeI y'GGCC_r
Cuts at: 0 237 596 612
Size: 237 359 16
Fragments arranged by size:
359 237 16
EagI C'GGCC_G
Cuts at: 0 237 612
Size: 237 375
EcoICRI GAG'CTC
Cuts at: 0 528 612
Size: 528 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 612
Size: 607 5
EcoO109I rG'GnC_Cy
Cuts at: 0 330 612
Size: 330 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 303 612
Size: 303 309
FspI TGC'GCA
Cuts at: 0 271 612
Size: 271 341
FspAI rTGC'GCAy
Cuts at: 0 271 612
Size: 271 341
HaeII r_GCGC'y
Cuts at: 0 213 473 612
Size: 213 260 139
Fragments arranged by size:
260 213 139
MscI TGG'CCA
Cuts at: 0 598 612
Size: 598 14
MslI CAynn'nnrTG
Cuts at: 0 118 229 265 612
Size: 118 111 36 347
Fragments arranged by size:
347 118 111 36
MspA1I CmG'CkG
Cuts at: 0 28 612
Size: 28 584
NspI r_CATG'y
Cuts at: 0 286 612
Size: 286 326
PpuMI rG'GwC_Cy
Cuts at: 0 330 612
Size: 330 282
PstI C_TGCA'G
Cuts at: 0 107 329 612
Size: 107 222 283
Fragments arranged by size:
283 222 107
SacI G_AGCT'C
Cuts at: 0 530 612
Size: 530 82
SbfI CC_TGCA'GG
Cuts at: 0 107 612
Size: 107 505
SfcI C'TryA_G
Cuts at: 0 103 325 612
Size: 103 222 287
Fragments arranged by size:
287 222 103
SmaI CCC'GGG
Cuts at: 0 364 612
Size: 364 248
SphI G_CATG'C
Cuts at: 0 286 612
Size: 286 326
StyI C'CwwG_G
Cuts at: 0 542 612
Size: 542 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 612
Size: 19 593
XmaI C'CCGG_G
Cuts at: 0 362 612
Size: 362 250
Enzymes that do cut:
AarI AlwNI ApaLI AscI AvaI BanII Bme1580I BpmI
BsaI BsaWI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BssHII BssSI BtsI CspCI
EaeI EagI EcoICRI Eco57MI EcoO109I FalI FspI FspAI
HaeII MscI MslI MspA1I NspI PpuMI PstI SacI
SbfI SfcI SmaI SphI StyI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApoI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaXI BseYI BsiWI BsmI BsrBI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EarI EciI EcoNI EcoRI
EcoRV FseI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI