(Linear) (Six-base) MAPSORT of: KIEE5215.seq  Check: 9494  from: 1  to: 723



KIEE5215.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:51  ..

AccI GT'mk_AC

Cuts at:      0     574     723 
   Size:        574     149
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     635     723 
   Size:        311     324      88

  Fragments arranged by size: 

                 324     311      88
 
ApoI r'AATT_y

Cuts at:      0     276     723 
   Size:        276     447
 
BanI G'GyrC_C

Cuts at:      0     665     723 
   Size:        665      58
 
BanII G_rGCy'C

Cuts at:      0     701     723 
   Size:        701      22
 
BbeI G_GCGC'C

Cuts at:      0     669     723 
   Size:        669      54
 
BbvCI CC'TCA_GC

Cuts at:      0       4     723 
   Size:          4     719
 
BglII A'GATC_T

Cuts at:      0      42     723 
   Size:         42     681
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     454     723 
   Size:        454     269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     723 
   Size:        431     292
 
Bpu10I CC'TnA_GC

Cuts at:      0       4     723 
   Size:          4     719
 
BsaHI Gr'CG_yC

Cuts at:      0     666     723 
   Size:        666      57
 
BseYI C'CCAG_C

Cuts at:      0     416     580     723 
   Size:        416     164     143

  Fragments arranged by size: 

                 416     164     143
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     723 
   Size:        391     332
 
BsiEI CG_ry'CG

Cuts at:      0     683     723 
   Size:        683      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     701     723 
   Size:        701      22
 
BssHII G'CGCG_C

Cuts at:      0     716     723 
   Size:        716       7
 
BssSI C'ACGA_G

Cuts at:      0     182     723 
   Size:        182     541
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     467     588     723 
   Size:        467     121     135

  Fragments arranged by size: 

                 467     135     121
 
BstYI r'GATC_y

Cuts at:      0      42     723 
   Size:         42     681
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     502     723 
   Size:        502     221
 
BtsI GCAGTG_nn'

Cuts at:      0     176     723 
   Size:        176     547
 
DraI TTT'AAA

Cuts at:      0     252     723 
   Size:        252     471
 
EarI CTCTTCn'nnn_

Cuts at:      0      59      68     217     258     611     620     723 
   Size:         59       9     149      41     353       9     103

  Fragments arranged by size: 

                 353     149     103      59      41       9       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     431     635     723 
   Size:        311     120     204      88

  Fragments arranged by size: 

                 311     204     120      88
 
HaeII r_GCGC'y

Cuts at:      0     669     723 
   Size:        669      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      22      54     723 
   Size:         22      32     669

  Fragments arranged by size: 

                 669      32      22
 
HincII GTy'rAC

Cuts at:      0     575     723 
   Size:        575     148
 
HindIII A'AGCT_T

Cuts at:      0     255     723 
   Size:        255     468
 
KasI G'GCGC_C

Cuts at:      0     665     723 
   Size:        665      58
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     703     723 
   Size:        703      20
 
MslI CAynn'nnrTG

Cuts at:      0     599     723 
   Size:        599     124
 
MspA1I CmG'CkG

Cuts at:      0     246     723 
   Size:        246     477
 
NarI GG'CG_CC

Cuts at:      0     666     723 
   Size:        666      57
 
PsiI TTA'TAA

Cuts at:      0     499     723 
   Size:        499     224
 
PvuII CAG'CTG

Cuts at:      0     246     723 
   Size:        246     477
 
SalI G'TCGA_C

Cuts at:      0     573     723 
   Size:        573     150
 
SexAI A'CCwGG_T

Cuts at:      0     230     723 
   Size:        230     493
 
SfoI GGC'GCC

Cuts at:      0     667     723 
   Size:        667      56
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     594     723 
   Size:        594     129
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BanI    BanII     BbeI    BbvCI    BglII
     BmrI     BpmI   Bpu10I    BsaHI    BseYI     BsgI    BsiEI Bsp1286I
   BssHII    BssSI    BstXI    BstYI    BtgZI     BtsI     DraI     EarI
  Eco57MI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MslI
   MspA1I     NarI     PsiI    PvuII     SalI    SexAI     SfoI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI     HpaI     KpnI     MfeI     MluI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI