(Linear) (Six-base) MAPSORT of: KIEE5213.seq  Check: 5583  from: 1  to: 732



KIEE5213.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:51  ..

AflII C'TTAA_G

Cuts at:      0     454     732 
   Size:        454     278
 
AhdI GACnn_n'nnGTC

Cuts at:      0      14     732 
   Size:         14     718
 
AleI CACnn'nnGTG

Cuts at:      0      37     732 
   Size:         37     695
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     732 
   Size:         18     714
 
BglII A'GATC_T

Cuts at:      0     404     732 
   Size:        404     328
 
BlpI GC'TnA_GC

Cuts at:      0     700     732 
   Size:        700      32
 
BmtI G_CTAG'C

Cuts at:      0     169     732 
   Size:        169     563
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     522     732 
   Size:         81     441     210

  Fragments arranged by size: 

                 441     210      81
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     732 
   Size:        268     464
 
BsaWI w'CCGG_w

Cuts at:      0     311     732 
   Size:        311     421
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     474     732 
   Size:        474     258
 
BseYI C'CCAG_C

Cuts at:      0      21     625     732 
   Size:         21     604     107

  Fragments arranged by size: 

                 604     107      21
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     465     732 
   Size:        465     267
 
BsmI GAATG_Cn'

Cuts at:      0     449     732 
   Size:        449     283
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     656     732 
   Size:        656      76
 
BsrGI T'GTAC_A

Cuts at:      0     321     732 
   Size:        321     411
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      18     732 
   Size:         18     714
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     232     732 
   Size:        232     500
 
BstYI r'GATC_y

Cuts at:      0      92     185     198     404     732 
   Size:         92      93      13     206     328

  Fragments arranged by size: 

                 328     206      93      92      13
 
Bsu36I CC'TnA_GG

Cuts at:      0     241     670     732 
   Size:        241     429      62

  Fragments arranged by size: 

                 429     241      62
 
BtsI GCAGTG_nn'

Cuts at:      0      19     477     732 
   Size:         19     458     255

  Fragments arranged by size: 

                 458     255      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     172     732 
   Size:        172     560
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      81     522     732 
   Size:         81     441     210

  Fragments arranged by size: 

                 441     210      81
 
EcoO109I rG'GnC_Cy

Cuts at:      0     237     332     510     732 
   Size:        237      95     178     222

  Fragments arranged by size: 

                 237     222     178      95
 
EcoRV GAT'ATC

Cuts at:      0     132     732 
   Size:        132     600
 
FspI TGC'GCA

Cuts at:      0      31     732 
   Size:         31     701
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32     116     148     732 
   Size:         32      84      32     584

  Fragments arranged by size: 

                 584      84      32      32
 
MslI CAynn'nnrTG

Cuts at:      0      37     230     732 
   Size:         37     193     502

  Fragments arranged by size: 

                 502     193      37
 
MspA1I CmG'CkG

Cuts at:      0     682     732 
   Size:        682      50
 
NheI G'CTAG_C

Cuts at:      0     165     732 
   Size:        165     567
 
PasI CC'CwG_GG

Cuts at:      0     565     732 
   Size:        565     167
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      59     732 
   Size:         59     673
 
PfoI T'CCnGG_A

Cuts at:      0     154     350     551     732 
   Size:        154     196     201     181

  Fragments arranged by size: 

                 201     196     181     154
 
PpuMI rG'GwC_Cy

Cuts at:      0     237     510     732 
   Size:        237     273     222

  Fragments arranged by size: 

                 273     237     222
 
SmlI C'TyrA_G

Cuts at:      0     283     454     732 
   Size:        283     171     278

  Fragments arranged by size: 

                 283     278     171
 
TatI w'GTAC_w

Cuts at:      0     321     732 
   Size:        321     411
 

 Enzymes that do cut:

    AflII     AhdI     AleI    AlwNI    BglII     BlpI     BmtI     BpmI
    BpuEI    BsaWI    BseRI    BseYI     BsgI     BsmI    BspMI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I     BtsI     EarI  Eco57MI EcoO109I
    EcoRV     FspI    Hin4I     MslI   MspA1I     NheI     PasI    PflMI
     PfoI    PpuMI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI