(Linear) (Six-base) MAPSORT of: KIEE5211.seq  Check: 6507  from: 1  to: 621



KIEE5211.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AccI GT'mk_AC

Cuts at:      0     251     621 
   Size:        251     370
 
AlwNI CAG_nnn'CTG

Cuts at:      0      68     603     621 
   Size:         68     535      18

  Fragments arranged by size: 

                 535      68      18
 
ApaLI G'TGCA_C

Cuts at:      0     588     621 
   Size:        588      33
 
AvaI C'yCGr_G

Cuts at:      0     572     621 
   Size:        572      49
 
BanII G_rGCy'C

Cuts at:      0      17     621 
   Size:         17     604
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     274     621 
   Size:        274     347
 
BlpI GC'TnA_GC

Cuts at:      0     396     401     621 
   Size:        396       5     220

  Fragments arranged by size: 

                 396     220       5
 
Bme1580I G_kGCm'C

Cuts at:      0     592     621 
   Size:        592      29
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     416     512     621 
   Size:        416      96     109

  Fragments arranged by size: 

                 416     109      96
 
Bpu10I CC'TnA_GC

Cuts at:      0     391     621 
   Size:        391     230
 
BsaWI w'CCGG_w

Cuts at:      0     242     621 
   Size:        242     379
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     325     511     621 
   Size:        325     186     110

  Fragments arranged by size: 

                 325     186     110
 
BseYI C'CCAG_C

Cuts at:      0      58     621 
   Size:         58     563
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     621 
   Size:         54     567
 
BsiHKAI G_wGCw'C

Cuts at:      0     592     621 
   Size:        592      29
 
BsmI GAATG_Cn'

Cuts at:      0     451     621 
   Size:        451     170
 
Bsp1286I G_dGCh'C

Cuts at:      0      17     592     621 
   Size:         17     575      29

  Fragments arranged by size: 

                 575      29      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     370     621 
   Size:        370     251
 
BsrDI GCAATG_nn'

Cuts at:      0     320     498     621 
   Size:        320     178     123

  Fragments arranged by size: 

                 320     178     123
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      68     500     603     621 
   Size:         68     432     103      18

  Fragments arranged by size: 

                 432     103      68      18
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     138     621 
   Size:        138     483
 
Bsu36I CC'TnA_GG

Cuts at:      0     222     621 
   Size:        222     399
 
BtgI C'CryG_G

Cuts at:      0     263     621 
   Size:        263     358
 
EcoO109I rG'GnC_Cy

Cuts at:      0      65     293     621 
   Size:         65     228     328

  Fragments arranged by size: 

                 328     228      65
 
HaeII r_GCGC'y

Cuts at:      0     408     621 
   Size:        408     213
 
HincII GTy'rAC

Cuts at:      0     156     621 
   Size:        156     465
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     621 
   Size:        137     484
 
MspA1I CmG'CkG

Cuts at:      0     235     621 
   Size:        235     386
 
NcoI C'CATG_G

Cuts at:      0     263     621 
   Size:        263     358
 
PpuMI rG'GwC_Cy

Cuts at:      0     293     621 
   Size:        293     328
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     196     228     621 
   Size:        196      32     393

  Fragments arranged by size: 

                 393     196      32
 
PstI C_TGCA'G

Cuts at:      0     338     367     488     621 
   Size:        338      29     121     133

  Fragments arranged by size: 

                 338     133     121      29
 
SfcI C'TryA_G

Cuts at:      0     334     363     484     621 
   Size:        334      29     121     137

  Fragments arranged by size: 

                 334     137     121      29
 
StyI C'CwwG_G

Cuts at:      0     263     525     621 
   Size:        263     262      96

  Fragments arranged by size: 

                 263     262      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     104     621 
   Size:        104     517
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     441     473     621 
   Size:        441      32     148

  Fragments arranged by size: 

                 441     148      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     118     621 
   Size:        118     503
 

 Enzymes that do cut:

     AccI    AlwNI    ApaLI     AvaI    BanII    BciVI     BlpI Bme1580I
     BplI   Bpu10I    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI   BstAPI    BstXI   Bsu36I     BtgI EcoO109I
    HaeII   HincII     MmeI   MspA1I     NcoI    PpuMI     PsrI     PstI
     SfcI     StyI    TaqII     TstI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI