(Linear) (Six-base) MAPSORT of: KIEE5210.seq  Check: 6118  from: 1  to: 540



KIEE5210.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AccI GT'mk_AC

Cuts at:      0     467     540 
   Size:        467      73
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     540 
   Size:         35     505
 
ApoI r'AATT_y

Cuts at:      0     214     540 
   Size:        214     326
 
BanI G'GyrC_C

Cuts at:      0      97     540 
   Size:         97     443
 
BmgBI CAC'GTC

Cuts at:      0     246     540 
   Size:        246     294
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      16     540 
   Size:         16     524
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     540 
   Size:         34     506
 
BseYI C'CCAG_C

Cuts at:      0     195     540 
   Size:        195     345
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     326     540 
   Size:        100     226     214

  Fragments arranged by size: 

                 226     214     100
 
BsiEI CG_ry'CG

Cuts at:      0     126     540 
   Size:        126     414
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     523     540 
   Size:        523      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     211     540 
   Size:        211     329
 
BsrFI r'CCGG_y

Cuts at:      0      86     540 
   Size:         86     454
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     274     540 
   Size:        274     266
 
BtgI C'CryG_G

Cuts at:      0     404     473     540 
   Size:        404      69      67

  Fragments arranged by size: 

                 404      69      67
 
EaeI y'GGCC_r

Cuts at:      0     161     540 
   Size:        161     379
 
EarI CTCTTCn'nnn_

Cuts at:      0      11     521     540 
   Size:         11     510      19

  Fragments arranged by size: 

                 510      19      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      35     540 
   Size:         35     505
 
EcoO109I rG'GnC_Cy

Cuts at:      0     200     540 
   Size:        200     340
 
HincII GTy'rAC

Cuts at:      0     536     540 
   Size:        536       4
 
NcoI C'CATG_G

Cuts at:      0     404     473     540 
   Size:        404      69      67

  Fragments arranged by size: 

                 404      69      67
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     390     540 
   Size:        390     150
 
PpuMI rG'GwC_Cy

Cuts at:      0     200     540 
   Size:        200     340
 
PshAI GACnn'nnGTC

Cuts at:      0      28     540 
   Size:         28     512
 
PstI C_TGCA'G

Cuts at:      0     460     540 
   Size:        460      80
 
SfcI C'TryA_G

Cuts at:      0     456     540 
   Size:        456      84
 
SspI AAT'ATT

Cuts at:      0     171     540 
   Size:        171     369
 
StyI C'CwwG_G

Cuts at:      0     404     473     540 
   Size:        404      69      67

  Fragments arranged by size: 

                 404      69      67
 
TatI w'GTAC_w

Cuts at:      0     131     398     540 
   Size:        131     267     142

  Fragments arranged by size: 

                 267     142     131
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     480     540 
   Size:        480      60
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BanI    BmgBI     BsaI    BseRI    BseYI
     BsgI    BsiEI    BsmBI    BspMI    BsrFI   BstAPI     BtgI     EaeI
     EarI  Eco57MI EcoO109I   HincII     NcoI    PflMI    PpuMI    PshAI
     PstI     SfcI     SspI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI  BsiHKAI    BsiWI     BsmI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI