Restriction map for
pFN21AE5206
(Linear) (Six-base) MAPSORT of: KIEE5206.seq Check: 5766 from: 1 to: 753
KIEE5206.orf
With 182 enzymes: SgfI *
January 29, 2014 11:50 ..
AatII G_ACGT'C
Cuts at: 0 254 753
Size: 254 499
Acc65I G'GTAC_C
Cuts at: 0 647 753
Size: 647 106
AhdI GACnn_n'nnGTC
Cuts at: 0 507 753
Size: 507 246
AleI CACnn'nnGTG
Cuts at: 0 157 753
Size: 157 596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 416 580 753
Size: 416 164 173
Fragments arranged by size:
416 173 164
AvaI C'yCGr_G
Cuts at: 0 40 753
Size: 40 713
BanI G'GyrC_C
Cuts at: 0 647 753
Size: 647 106
BanII G_rGCy'C
Cuts at: 0 53 753
Size: 53 700
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 506 753
Size: 250 256 247
Fragments arranged by size:
256 250 247
BbvCI CC'TCA_GC
Cuts at: 0 742 753
Size: 742 11
BmgBI CAC'GTC
Cuts at: 0 240 753
Size: 240 513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 753
Size: 295 458
Bpu10I CC'TnA_GC
Cuts at: 0 742 753
Size: 742 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 753
Size: 637 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 274 753
Size: 274 479
BsaHI Gr'CG_yC
Cuts at: 0 251 482 753
Size: 251 231 271
Fragments arranged by size:
271 251 231
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 292 487 753
Size: 280 12 195 266
Fragments arranged by size:
280 266 195 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 713 753
Size: 172 541 40
Fragments arranged by size:
541 172 40
BsiHKAI G_wGCw'C
Cuts at: 0 53 753
Size: 53 700
Bsp1286I G_dGCh'C
Cuts at: 0 53 753
Size: 53 700
BspHI T'CATG_A
Cuts at: 0 116 753
Size: 116 637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 753
Size: 194 559
BstYI r'GATC_y
Cuts at: 0 162 753
Size: 162 591
ClaI AT'CG_AT
Cuts at: 0 71 753
Size: 71 682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 474 509 753
Size: 474 35 244
Fragments arranged by size:
474 244 35
EaeI y'GGCC_r
Cuts at: 0 593 753
Size: 593 160
EarI CTCTTCn'nnn_
Cuts at: 0 118 753
Size: 118 635
EcoICRI GAG'CTC
Cuts at: 0 51 753
Size: 51 702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 753
Size: 295 458
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 753
Size: 424 329
EcoO109I rG'GnC_Cy
Cuts at: 0 36 509 753
Size: 36 473 244
Fragments arranged by size:
473 244 36
EcoRV GAT'ATC
Cuts at: 0 618 753
Size: 618 135
HaeII r_GCGC'y
Cuts at: 0 111 753
Size: 111 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 493 525 753
Size: 68 32 393 32 228
Fragments arranged by size:
393 228 68 32 32
KpnI G_GTAC'C
Cuts at: 0 651 753
Size: 651 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 753
Size: 356 397
MscI TGG'CCA
Cuts at: 0 595 753
Size: 595 158
MslI CAynn'nnrTG
Cuts at: 0 157 753
Size: 157 596
MspA1I CmG'CkG
Cuts at: 0 181 340 697 753
Size: 181 159 357 56
Fragments arranged by size:
357 181 159 56
PfoI T'CCnGG_A
Cuts at: 0 272 524 753
Size: 272 252 229
Fragments arranged by size:
272 252 229
PpuMI rG'GwC_Cy
Cuts at: 0 36 509 753
Size: 36 473 244
Fragments arranged by size:
473 244 36
PshAI GACnn'nnGTC
Cuts at: 0 250 753
Size: 250 503
PvuII CAG'CTG
Cuts at: 0 181 697 753
Size: 181 516 56
Fragments arranged by size:
516 181 56
SacI G_AGCT'C
Cuts at: 0 53 753
Size: 53 700
SanDI GG'GwC_CC
Cuts at: 0 36 509 753
Size: 36 473 244
Fragments arranged by size:
473 244 36
SmlI C'TyrA_G
Cuts at: 0 652 753
Size: 652 101
StuI AGG'CCT
Cuts at: 0 419 638 753
Size: 419 219 115
Fragments arranged by size:
419 219 115
StyI C'CwwG_G
Cuts at: 0 425 753
Size: 425 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 313 345 753
Size: 313 32 408
Fragments arranged by size:
408 313 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 576 753
Size: 576 177
ZraI GAC'GTC
Cuts at: 0 252 753
Size: 252 501
Enzymes that do cut:
AatII Acc65I AhdI AleI AlfI AvaI BanI BanII
BbsI BbvCI BmgBI BpmI Bpu10I BpuEI BsaI BsaHI
BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BstYI ClaI
CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII Hin4I KpnI MmeI MscI MslI MspA1I PfoI
PpuMI PshAI PvuII SacI SanDI SmlI StuI StyI
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BsaAI
BsaBI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EagI EciI EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI