Restriction map for
pFN21AE5205
(Linear) (Six-base) MAPSORT of: KIEE5205.seq Check: 9731 from: 1 to: 720
KIEE5205.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 720
Size: 545 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 720
Size: 31 32 657
Fragments arranged by size:
657 32 31
ApaI G_GGCC'C
Cuts at: 0 178 720
Size: 178 542
BanII G_rGCy'C
Cuts at: 0 178 347 611 720
Size: 178 169 264 109
Fragments arranged by size:
264 178 169 109
BbvCI CC'TCA_GC
Cuts at: 0 686 720
Size: 686 34
Bme1580I G_kGCm'C
Cuts at: 0 178 720
Size: 178 542
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 630 720
Size: 630 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 720
Size: 505 215
Bpu10I CC'TnA_GC
Cuts at: 0 686 720
Size: 686 34
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 480 547 664 720
Size: 480 67 117 56
Fragments arranged by size:
480 117 67 56
BsmI GAATG_Cn'
Cuts at: 0 166 387 720
Size: 166 221 333
Fragments arranged by size:
333 221 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 720
Size: 154 566
Bsp1286I G_dGCh'C
Cuts at: 0 178 347 611 720
Size: 178 169 264 109
Fragments arranged by size:
264 178 169 109
BspHI T'CATG_A
Cuts at: 0 708 720
Size: 708 12
BsrFI r'CCGG_y
Cuts at: 0 14 78 720
Size: 14 64 642
Fragments arranged by size:
642 64 14
BssSI C'ACGA_G
Cuts at: 0 157 720
Size: 157 563
Bsu36I CC'TnA_GG
Cuts at: 0 101 720
Size: 101 619
EaeI y'GGCC_r
Cuts at: 0 76 618 720
Size: 76 542 102
Fragments arranged by size:
542 102 76
EarI CTCTTCn'nnn_
Cuts at: 0 193 323 457 641 653 659 720
Size: 193 130 134 184 12 6 61
Fragments arranged by size:
193 184 134 130 61 12 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 545 720
Size: 505 40 175
Fragments arranged by size:
505 175 40
EcoO109I rG'GnC_Cy
Cuts at: 0 39 152 175 566 625 690 720
Size: 39 113 23 391 59 65 30
Fragments arranged by size:
391 113 65 59 39 30 23
FseI GG_CCGG'CC
Cuts at: 0 82 720
Size: 82 638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 720
Size: 35 685
NaeI GCC'GGC
Cuts at: 0 80 720
Size: 80 640
NgoMIV G'CCGG_C
Cuts at: 0 78 720
Size: 78 642
NruI TCG'CGA
Cuts at: 0 6 720
Size: 6 714
PpuMI rG'GwC_Cy
Cuts at: 0 39 152 720
Size: 39 113 568
Fragments arranged by size:
568 113 39
PspOMI G'GGCC_C
Cuts at: 0 174 720
Size: 174 546
PstI C_TGCA'G
Cuts at: 0 357 720
Size: 357 363
SanDI GG'GwC_CC
Cuts at: 0 152 720
Size: 152 568
SapI GCTCTTCn'nnn_
Cuts at: 0 323 720
Size: 323 397
SexAI A'CCwGG_T
Cuts at: 0 41 720
Size: 41 679
SfcI C'TryA_G
Cuts at: 0 353 720
Size: 353 367
StyI C'CwwG_G
Cuts at: 0 206 434 506 551 674 720
Size: 206 228 72 45 123 46
Fragments arranged by size:
228 206 123 72 46 45
TatI w'GTAC_w
Cuts at: 0 26 720
Size: 26 694
XmnI GAAnn'nnTTC
Cuts at: 0 241 720
Size: 241 479
Enzymes that do cut:
AcuI AloI ApaI BanII BbvCI Bme1580I BplI BpmI
Bpu10I BseRI BsmI BsmBI Bsp1286I BspHI BsrFI BssSI
Bsu36I EaeI EarI Eco57MI EcoO109I FseI MmeI NaeI
NgoMIV NruI PpuMI PspOMI PstI SanDI SapI SexAI
SfcI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BspEI
BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI