(Linear) (Six-base) MAPSORT of: KIEE5202.seq  Check: 2571  from: 1  to: 615



KIEE5202.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

ApoI r'AATT_y

Cuts at:      0     547     587     615 
   Size:        547      40      28

  Fragments arranged by size: 

                 547      40      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     602     615 
   Size:        602      13
 
BmtI G_CTAG'C

Cuts at:      0     370     615 
   Size:        370     245
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     386     615 
   Size:        386     229
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      44     615 
   Size:         44     571
 
EaeI y'GGCC_r

Cuts at:      0     336     615 
   Size:        336     279
 
EarI CTCTTCn'nnn_

Cuts at:      0     239     347     615 
   Size:        239     108     268

  Fragments arranged by size: 

                 268     239     108
 
EcoRI G'AATT_C

Cuts at:      0     587     615 
   Size:        587      28
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     166     615 
   Size:        166     449
 
HincII GTy'rAC

Cuts at:      0     180     615 
   Size:        180     435
 
HindIII A'AGCT_T

Cuts at:      0     156     615 
   Size:        156     459
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     615 
   Size:        236     379
 
NheI G'CTAG_C

Cuts at:      0     366     615 
   Size:        366     249
 
NspI r_CATG'y

Cuts at:      0     301     615 
   Size:        301     314
 
SapI GCTCTTCn'nnn_

Cuts at:      0     239     347     615 
   Size:        239     108     268

  Fragments arranged by size: 

                 268     239     108
 
SfcI C'TryA_G

Cuts at:      0     307     615 
   Size:        307     308
 
SphI G_CATG'C

Cuts at:      0     301     615 
   Size:        301     314
 
SspI AAT'ATT

Cuts at:      0      56     615 
   Size:         56     559
 
XmnI GAAnn'nnTTC

Cuts at:      0     149     615 
   Size:        149     466
 

 Enzymes that do cut:

     ApoI     BbsI     BmtI     BsaI     BsgI     EaeI     EarI    EcoRI
     FalI   HincII  HindIII     MmeI     NheI     NspI     SapI     SfcI
     SphI     SspI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI