(Linear) (Six-base) MAPSORT of: KIEE5201.seq  Check: 7894  from: 1  to: 654



KIEE5201.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     579     654 
   Size:        325     254      75

  Fragments arranged by size: 

                 325     254      75
 
AhdI GACnn_n'nnGTC

Cuts at:      0     140     654 
   Size:        140     514
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     442     654 
   Size:        442     212
 
ApoI r'AATT_y

Cuts at:      0     217     545     654 
   Size:        217     328     109

  Fragments arranged by size: 

                 328     217     109
 
AvaI C'yCGr_G

Cuts at:      0     149     654 
   Size:        149     505
 
BbvCI CC'TCA_GC

Cuts at:      0      86     654 
   Size:         86     568
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     379     654 
   Size:        379     275
 
Bpu10I CC'TnA_GC

Cuts at:      0      86     654 
   Size:         86     568
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     654 
   Size:        187     467
 
BsaBI GATnn'nnATC

Cuts at:      0     605     654 
   Size:        605      49
 
BsaHI Gr'CG_yC

Cuts at:      0      20     654 
   Size:         20     634
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     654 
   Size:        203     451
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     654 
   Size:        203     451
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     239     654 
   Size:        239     415
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     435     654 
   Size:        435     219
 
BstYI r'GATC_y

Cuts at:      0     271     654 
   Size:        271     383
 
DraIII CAC_nnn'GTG

Cuts at:      0     511     654 
   Size:        511     143
 
EarI CTCTTCn'nnn_

Cuts at:      0     301     654 
   Size:        301     353
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325     579     654 
   Size:        325     254      75

  Fragments arranged by size: 

                 325     254      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     447     654 
   Size:        447     207
 
EcoO109I rG'GnC_Cy

Cuts at:      0     500     654 
   Size:        500     154
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     405     546     654 
   Size:        405     141     108

  Fragments arranged by size: 

                 405     141     108
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     126     158     349     381     654 
   Size:        126      32     191      32     273

  Fragments arranged by size: 

                 273     191     126      32      32
 
HincII GTy'rAC

Cuts at:      0     329     654 
   Size:        329     325
 
HindIII A'AGCT_T

Cuts at:      0     483     654 
   Size:        483     171
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     495     516     654 
   Size:        495      21     138

  Fragments arranged by size: 

                 495     138      21
 
MslI CAynn'nnrTG

Cuts at:      0     280     614     654 
   Size:        280     334      40

  Fragments arranged by size: 

                 334     280      40
 
PpuMI rG'GwC_Cy

Cuts at:      0     500     654 
   Size:        500     154
 
SapI GCTCTTCn'nnn_

Cuts at:      0     301     654 
   Size:        301     353
 
SexAI A'CCwGG_T

Cuts at:      0      42     654 
   Size:         42     612
 
SfcI C'TryA_G

Cuts at:      0     287     654 
   Size:        287     367
 
SmlI C'TyrA_G

Cuts at:      0     149     202     654 
   Size:        149      53     452

  Fragments arranged by size: 

                 452     149      53
 
StyI C'CwwG_G

Cuts at:      0     443     654 
   Size:        443     211
 
XhoI C'TCGA_G

Cuts at:      0     149     654 
   Size:        149     505
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI     ApoI     AvaI    BbvCI     BplI   Bpu10I
    BpuEI    BsaBI    BsaHI  BsiHKAI Bsp1286I    BspMI   BstAPI    BstYI
   DraIII     EarI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII
  HindIII     MmeI     MslI    PpuMI     SapI    SexAI     SfcI     SmlI
     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI     BsaI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI