(Linear) (Six-base) MAPSORT of: KIEE5195.seq  Check: 879  from: 1  to: 468



KIEE5195.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     253     468 
   Size:        253     215
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      39     468 
   Size:         39     429
 
ApaI G_GGCC'C

Cuts at:      0     308     468 
   Size:        308     160
 
ApoI r'AATT_y

Cuts at:      0     119     468 
   Size:        119     349
 
BanI G'GyrC_C

Cuts at:      0       3     468 
   Size:          3     465
 
BanII G_rGCy'C

Cuts at:      0     308     468 
   Size:        308     160
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     356     468 
   Size:        356     112
 
Bme1580I G_kGCm'C

Cuts at:      0     308     468 
   Size:        308     160
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      67     468 
   Size:         67     401
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     243     468 
   Size:        243     225
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     468 
   Size:        213     255
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     468 
   Size:        128     340
 
BsaAI yAC'GTr

Cuts at:      0     390     468 
   Size:        390      78
 
BseYI C'CCAG_C

Cuts at:      0      48     136     468 
   Size:         48      88     332

  Fragments arranged by size: 

                 332      88      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     468 
   Size:        199     269
 
Bsp1286I G_dGCh'C

Cuts at:      0     308     468 
   Size:        308     160
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     253     347     468 
   Size:        253      94     121

  Fragments arranged by size: 

                 253     121      94
 
BsrFI r'CCGG_y

Cuts at:      0       6     468 
   Size:          6     462
 
BsrGI T'GTAC_A

Cuts at:      0     447     468 
   Size:        447      21
 
BssSI C'ACGA_G

Cuts at:      0     217     468 
   Size:        217     251
 
BstYI r'GATC_y

Cuts at:      0      68     468 
   Size:         68     400
 
Bsu36I CC'TnA_GG

Cuts at:      0     336     468 
   Size:        336     132
 
BtgI C'CryG_G

Cuts at:      0     300     468 
   Size:        300     168
 
DraI TTT'AAA

Cuts at:      0     117     468 
   Size:        117     351
 
EaeI y'GGCC_r

Cuts at:      0       8     236     468 
   Size:          8     228     232

  Fragments arranged by size: 

                 232     228       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213     468 
   Size:        213     255
 
MslI CAynn'nnrTG

Cuts at:      0     108     468 
   Size:        108     360
 
NspI r_CATG'y

Cuts at:      0     132     152     468 
   Size:        132      20     316

  Fragments arranged by size: 

                 316     132      20
 
PspOMI G'GGCC_C

Cuts at:      0     304     468 
   Size:        304     164
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     343     370     375     402     468 
   Size:        343      27       5      27      66

  Fragments arranged by size: 

                 343      66      27      27       5
 
PstI C_TGCA'G

Cuts at:      0     358     468 
   Size:        358     110
 
SexAI A'CCwGG_T

Cuts at:      0     437     468 
   Size:        437      31
 
SfcI C'TryA_G

Cuts at:      0     354     468 
   Size:        354     114
 
SmlI C'TyrA_G

Cuts at:      0     143     468 
   Size:        143     325
 
SnaBI TAC'GTA

Cuts at:      0     390     468 
   Size:        390      78
 
SphI G_CATG'C

Cuts at:      0     152     468 
   Size:        152     316
 
StyI C'CwwG_G

Cuts at:      0      89     468 
   Size:         89     379
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      48     468 
   Size:         48     420
 
TatI w'GTAC_w

Cuts at:      0     447     468 
   Size:        447      21
 
Tth111I GACn'n_nGTC

Cuts at:      0     460     468 
   Size:        460       8
 

 Enzymes that do cut:

     AarI     AlfI     ApaI     ApoI     BanI    BanII    BciVI Bme1580I
     BmrI     BplI     BpmI    BpuEI    BsaAI    BseYI     BsgI Bsp1286I
    BspMI    BsrFI    BsrGI    BssSI    BstYI   Bsu36I     BtgI     DraI
     EaeI  Eco57MI     MslI     NspI   PspOMI     PsrI     PstI    SexAI
     SfcI     SmlI    SnaBI     SphI     StyI    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SpeI     SrfI     SspI     StuI     SwaI     TstI     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI