(Linear) (Six-base) MAPSORT of: KIEE5190.seq  Check: 889  from: 1  to: 396



KIEE5190.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AccI GT'mk_AC

Cuts at:      0      38     396 
   Size:         38     358
 
ApaLI G'TGCA_C

Cuts at:      0      48     396 
   Size:         48     348
 
BanI G'GyrC_C

Cuts at:      0      67     120     251     396 
   Size:         67      53     131     145

  Fragments arranged by size: 

                 145     131      67      53
 
BclI T'GATC_A

Cuts at:      0     140     396 
   Size:        140     256
 
Bme1580I G_kGCm'C

Cuts at:      0      52     396 
   Size:         52     344
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     303     396 
   Size:        303      93
 
Bpu10I CC'TnA_GC

Cuts at:      0     261     396 
   Size:        261     135
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     396 
   Size:        139     257
 
BsaWI w'CCGG_w

Cuts at:      0      52     396 
   Size:         52     344
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      47      77     396 
   Size:         47      30     319

  Fragments arranged by size: 

                 319      47      30
 
BseYI C'CCAG_C

Cuts at:      0     387     396 
   Size:        387       9
 
BsiHKAI G_wGCw'C

Cuts at:      0      52     396 
   Size:         52     344
 
BsmI GAATG_Cn'

Cuts at:      0     388     396 
   Size:        388       8
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     396 
   Size:         52     344
 
BspHI T'CATG_A

Cuts at:      0     345     396 
   Size:        345      51
 
BsrDI GCAATG_nn'

Cuts at:      0     233     396 
   Size:        233     163
 
BstYI r'GATC_y

Cuts at:      0     293     396 
   Size:        293     103
 
BstZ17I GTA'TAC

Cuts at:      0      39     396 
   Size:         39     357
 
Bsu36I CC'TnA_GG

Cuts at:      0      43     396 
   Size:         43     353
 
ClaI AT'CG_AT

Cuts at:      0     356     396 
   Size:        356      40
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     309     396 
   Size:        309      87
 
EaeI y'GGCC_r

Cuts at:      0     333     396 
   Size:        333      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      47      79     396 
   Size:         47      32     317

  Fragments arranged by size: 

                 317      47      32
 
HincII GTy'rAC

Cuts at:      0     231     396 
   Size:        231     165
 
MslI CAynn'nnrTG

Cuts at:      0     344     396 
   Size:        344      52
 
PasI CC'CwG_GG

Cuts at:      0     102     396 
   Size:        102     294
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     315     396 
   Size:        315      81
 
SmlI C'TyrA_G

Cuts at:      0     154     396 
   Size:        154     242
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      78     396 
   Size:         78     318
 

 Enzymes that do cut:

     AccI    ApaLI     BanI     BclI Bme1580I     BmrI   Bpu10I    BpuEI
    BsaWI    BsaXI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI    BsrDI
    BstYI  BstZ17I   Bsu36I     ClaI     DrdI     EaeI    Hin4I   HincII
     MslI     PasI    PflMI     SmlI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI    BsaBI
    BsaHI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI