(Linear) (Six-base) MAPSORT of: KIEE5186.seq  Check: 400  from: 1  to: 237



KIEE5186.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AflIII A'CryG_T

Cuts at:      0     151     237 
   Size:        151      86
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      94     237 
   Size:         94     143
 
ApoI r'AATT_y

Cuts at:      0     205     237 
   Size:        205      32
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     194     237 
   Size:        194      43
 
BsiEI CG_ry'CG

Cuts at:      0      11     237 
   Size:         11     226
 
BssSI C'ACGA_G

Cuts at:      0      91     237 
   Size:         91     146
 
EaeI y'GGCC_r

Cuts at:      0       8     237 
   Size:          8     229
 
EagI C'GGCC_G

Cuts at:      0       8     237 
   Size:          8     229
 
EarI CTCTTCn'nnn_

Cuts at:      0     171     237 
   Size:        171      66
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     237 
   Size:          2     235
 
SspI AAT'ATT

Cuts at:      0     109     237 
   Size:        109     128
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      59     237 
   Size:         59     178
 

 Enzymes that do cut:

   AflIII     AlfI     ApoI    BseRI    BsiEI    BssSI     EaeI     EagI
     EarI     MmeI     SspI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI