(Linear) (Six-base) MAPSORT of: KIEE5181.seq  Check: 2230  from: 1  to: 342



KIEE5181.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AleI CACnn'nnGTG

Cuts at:      0     226     342 
   Size:        226     116
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     342 
   Size:        117     225
 
BmgBI CAC'GTC

Cuts at:      0     237     342 
   Size:        237     105
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     342 
   Size:        183     159
 
BseYI C'CCAG_C

Cuts at:      0     110     305     342 
   Size:        110     195      37

  Fragments arranged by size: 

                 195     110      37
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     227     342 
   Size:        227     115
 
BsrFI r'CCGG_y

Cuts at:      0     120     342 
   Size:        120     222
 
BtsI GCAGTG_nn'

Cuts at:      0      41     342 
   Size:         41     301
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     342 
   Size:         89     253
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28     224     244     256     276     342 
   Size:         28     196      20      12      20      66

  Fragments arranged by size: 

                 196      66      28      20      20      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     342 
   Size:        287      55
 
MslI CAynn'nnrTG

Cuts at:      0     226     342 
   Size:        226     116
 
MspA1I CmG'CkG

Cuts at:      0     325     342 
   Size:        325      17
 
PpuMI rG'GwC_Cy

Cuts at:      0      89     342 
   Size:         89     253
 
PvuII CAG'CTG

Cuts at:      0     325     342 
   Size:        325      17
 
SmlI C'TyrA_G

Cuts at:      0     162     342 
   Size:        162     180
 
StyI C'CwwG_G

Cuts at:      0      24     342 
   Size:         24     318
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     216     342 
   Size:        216     126
 
Tth111I GACn'n_nGTC

Cuts at:      0     238     342 
   Size:        238     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     221     342 
   Size:        221     121
 

 Enzymes that do cut:

     AleI    AlwNI    BmgBI    BpuEI    BseYI    BsmBI    BsrFI     BtsI
 EcoO109I    Hin4I     MmeI     MslI   MspA1I    PpuMI    PvuII     SmlI
     StyI    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI