(Linear) (Six-base) MAPSORT of: KIEE5180.seq  Check: 8122  from: 1  to: 456



KIEE5180.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AccI GT'mk_AC

Cuts at:      0     197     456 
   Size:        197     259
 
AfeI AGC'GCT

Cuts at:      0     306     456 
   Size:        306     150
 
AlwNI CAG_nnn'CTG

Cuts at:      0     261     456 
   Size:        261     195
 
ApaI G_GGCC'C

Cuts at:      0      16     456 
   Size:         16     440
 
BanII G_rGCy'C

Cuts at:      0      16     456 
   Size:         16     440
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      27      55     456 
   Size:         27      28     401

  Fragments arranged by size: 

                 401      28      27
 
BglI GCCn_nnn'nGGC

Cuts at:      0     372     456 
   Size:        372      84
 
Bme1580I G_kGCm'C

Cuts at:      0      16     456 
   Size:         16     440
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     271     456 
   Size:        271     185
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     175     456 
   Size:        136      39     281

  Fragments arranged by size: 

                 281     136      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      94     456 
   Size:         94     362
 
BsaAI yAC'GTr

Cuts at:      0     231     456 
   Size:        231     225
 
BsaWI w'CCGG_w

Cuts at:      0     442     456 
   Size:        442      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     288     456 
   Size:        288     168
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     456 
   Size:         49     407
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     456 
   Size:         16     440
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      21     456 
   Size:         21     435
 
BsrDI GCAATG_nn'

Cuts at:      0      90     456 
   Size:         90     366
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      27     456 
   Size:         27     429
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     244     368     456 
   Size:        244     124      88

  Fragments arranged by size: 

                 244     124      88
 
BtsI GCAGTG_nn'

Cuts at:      0      69     456 
   Size:         69     387
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     205     456 
   Size:        205     251
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     175     456 
   Size:        136      39     281

  Fragments arranged by size: 

                 281     136      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0      12     456 
   Size:         12     444
 
HaeII r_GCGC'y

Cuts at:      0     308     456 
   Size:        308     148
 
MslI CAynn'nnrTG

Cuts at:      0      97     456 
   Size:         97     359
 
MspA1I CmG'CkG

Cuts at:      0      18      61     456 
   Size:         18      43     395

  Fragments arranged by size: 

                 395      43      18
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     261     456 
   Size:        261     195
 
PmlI CAC'GTG

Cuts at:      0     231     456 
   Size:        231     225
 
PspOMI G'GGCC_C

Cuts at:      0      12     456 
   Size:         12     444
 
PstI C_TGCA'G

Cuts at:      0      23     456 
   Size:         23     433
 
PvuII CAG'CTG

Cuts at:      0      61     456 
   Size:         61     395
 
SfcI C'TryA_G

Cuts at:      0      19     139     456 
   Size:         19     120     317

  Fragments arranged by size: 

                 317     120      19
 
StuI AGG'CCT

Cuts at:      0     296     456 
   Size:        296     160
 
StyI C'CwwG_G

Cuts at:      0       8     456 
   Size:          8     448
 
TatI w'GTAC_w

Cuts at:      0     143     456 
   Size:        143     313
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     368     456 
   Size:        368      88
 

 Enzymes that do cut:

     AccI     AfeI    AlwNI     ApaI    BanII     BbsI     BglI Bme1580I
     BmrI     BpmI     BsaI    BsaAI    BsaWI    BseRI     BsgI Bsp1286I
    BspMI    BsrDI   BstAPI    BstXI     BtsI     DrdI  Eco57MI EcoO109I
    HaeII     MslI   MspA1I    PflMI     PmlI   PspOMI     PstI    PvuII
     SfcI     StuI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI