(Linear) (Six-base) MAPSORT of: KIEE5179.seq  Check: 1699  from: 1  to: 330



KIEE5179.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

ApaI G_GGCC'C

Cuts at:      0     231     330 
   Size:        231      99
 
ApoI r'AATT_y

Cuts at:      0     118     259     330 
   Size:        118     141      71

  Fragments arranged by size: 

                 141     118      71
 
AvaI C'yCGr_G

Cuts at:      0     104     224     330 
   Size:        104     120     106

  Fragments arranged by size: 

                 120     106     104
 
BanII G_rGCy'C

Cuts at:      0     231     330 
   Size:        231      99
 
Bme1580I G_kGCm'C

Cuts at:      0     231     330 
   Size:        231      99
 
Bpu10I CC'TnA_GC

Cuts at:      0     281     330 
   Size:        281      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     330 
   Size:        306      24
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      55     330 
   Size:         55     275
 
BsaWI w'CCGG_w

Cuts at:      0      53     330 
   Size:         53     277
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     159     189     330 
   Size:        159      30     141

  Fragments arranged by size: 

                 159     141      30
 
BsiHKAI G_wGCw'C

Cuts at:      0      71     330 
   Size:         71     259
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     231     330 
   Size:         71     160      99

  Fragments arranged by size: 

                 160      99      71
 
BspEI T'CCGG_A

Cuts at:      0      53     330 
   Size:         53     277
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     137     330 
   Size:        137     193
 
BtgI C'CryG_G

Cuts at:      0     231     330 
   Size:        231      99
 
EarI CTCTTCn'nnn_

Cuts at:      0      83     330 
   Size:         83     247
 
EcoRI G'AATT_C

Cuts at:      0     118     330 
   Size:        118     212
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     284     316     330 
   Size:        284      32      14

  Fragments arranged by size: 

                 284      32      14
 
NcoI C'CATG_G

Cuts at:      0     231     330 
   Size:        231      99
 
PspOMI G'GGCC_C

Cuts at:      0     227     330 
   Size:        227     103
 
SapI GCTCTTCn'nnn_

Cuts at:      0      83     330 
   Size:         83     247
 
SmlI C'TyrA_G

Cuts at:      0     321     330 
   Size:        321       9
 
StyI C'CwwG_G

Cuts at:      0      20     231     330 
   Size:         20     211      99

  Fragments arranged by size: 

                 211      99      20
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     159     191     330 
   Size:        159      32     139

  Fragments arranged by size: 

                 159     139      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     238     330 
   Size:        238      92
 

 Enzymes that do cut:

     ApaI     ApoI     AvaI    BanII Bme1580I   Bpu10I    BpuEI     BsaI
    BsaWI    BsaXI  BsiHKAI Bsp1286I    BspEI   BstAPI     BtgI     EarI
    EcoRI    Hin4I     NcoI   PspOMI     SapI     SmlI     StyI     TstI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI    BsaAI    BsaBI
    BsaHI    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI