(Linear) (Six-base) MAPSORT of: KIEE5176.seq  Check: 7769  from: 1  to: 345



KIEE5176.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:00  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     159     289     345 
   Size:        159     130      56

  Fragments arranged by size: 

                 159     130      56
 
ApoI r'AATT_y

Cuts at:      0     103     345 
   Size:        103     242
 
BanI G'GyrC_C

Cuts at:      0      77     345 
   Size:         77     268
 
BlpI GC'TnA_GC

Cuts at:      0     290     345 
   Size:        290      55
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     124     345 
   Size:        124     221
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     345 
   Size:        136     209
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     345 
   Size:        244     101
 
BseYI C'CCAG_C

Cuts at:      0     339     345 
   Size:        339       6
 
BsmI GAATG_Cn'

Cuts at:      0     266     345 
   Size:        266      79
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      37     345 
   Size:         37     308
 
BsrBI CCG'CTC

Cuts at:      0     219     345 
   Size:        219     126
 
BsrDI GCAATG_nn'

Cuts at:      0     321     329     345 
   Size:        321       8      16

  Fragments arranged by size: 

                 321      16       8
 
BsrFI r'CCGG_y

Cuts at:      0     167     345 
   Size:        167     178
 
BstYI r'GATC_y

Cuts at:      0     188     345 
   Size:        188     157
 
EarI CTCTTCn'nnn_

Cuts at:      0     200     209     251     326     345 
   Size:        200       9      42      75      19

  Fragments arranged by size: 

                 200      75      42      19       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     345 
   Size:        136     209
 
EcoO109I rG'GnC_Cy

Cuts at:      0     336     345 
   Size:        336       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     228     260     345 
   Size:        228      32      85

  Fragments arranged by size: 

                 228      85      32
 
HincII GTy'rAC

Cuts at:      0     134     345 
   Size:        134     211
 
MspA1I CmG'CkG

Cuts at:      0     156     195     345 
   Size:        156      39     150

  Fragments arranged by size: 

                 156     150      39
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     198     345 
   Size:        198     147
 
PpuMI rG'GwC_Cy

Cuts at:      0     336     345 
   Size:        336       9
 
PstI C_TGCA'G

Cuts at:      0     279     345 
   Size:        279      66
 
PvuII CAG'CTG

Cuts at:      0     156     195     345 
   Size:        156      39     150

  Fragments arranged by size: 

                 156     150      39
 
SapI GCTCTTCn'nnn_

Cuts at:      0     209     251     345 
   Size:        209      42      94

  Fragments arranged by size: 

                 209      94      42
 
SfcI C'TryA_G

Cuts at:      0     275     345 
   Size:        275      70
 
XmnI GAAnn'nnTTC

Cuts at:      0     245     345 
   Size:        245     100
 

 Enzymes that do cut:

    AlwNI     ApoI     BanI     BlpI     BplI     BpmI    BseRI    BseYI
     BsmI    BsmBI    BsrBI    BsrDI    BsrFI    BstYI     EarI  Eco57MI
 EcoO109I    Hin4I   HincII   MspA1I    PflMI    PpuMI     PstI    PvuII
     SapI     SfcI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI Bsp1286I    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI