(Linear) (Six-base) MAPSORT of: KIEE5173.seq  Check: 9395  from: 1  to: 507



KIEE5173.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     507 
   Size:        448      59
 
AflIII A'CryG_T

Cuts at:      0      21     507 
   Size:         21     486
 
AvrII C'CTAG_G

Cuts at:      0     414     507 
   Size:        414      93
 
BamHI G'GATC_C

Cuts at:      0     392     507 
   Size:        392     115
 
BanII G_rGCy'C

Cuts at:      0      48     507 
   Size:         48     459
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     354     507 
   Size:        354     153
 
BbvCI CC'TCA_GC

Cuts at:      0      59     507 
   Size:         59     448
 
BglI GCCn_nnn'nGGC

Cuts at:      0     215     507 
   Size:        215     292
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     473     507 
   Size:        473      34
 
Bpu10I CC'TnA_GC

Cuts at:      0      59     507 
   Size:         59     448
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     188     249     507 
   Size:        188      61     258

  Fragments arranged by size: 

                 258     188      61
 
BsaBI GATnn'nnATC

Cuts at:      0     202     507 
   Size:        202     305
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     446     476     507 
   Size:        446      30      31

  Fragments arranged by size: 

                 446      31      30
 
BseYI C'CCAG_C

Cuts at:      0     437     507 
   Size:        437      70
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     507 
   Size:        257     250
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     507 
   Size:         48     459
 
BsrBI CCG'CTC

Cuts at:      0     117     507 
   Size:        117     390
 
BsrDI GCAATG_nn'

Cuts at:      0      33     507 
   Size:         33     474
 
BstYI r'GATC_y

Cuts at:      0     392     507 
   Size:        392     115
 
BtgI C'CryG_G

Cuts at:      0      95     191     213     507 
   Size:         95      96      22     294

  Fragments arranged by size: 

                 294      96      95      22
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     211     507 
   Size:        211     296
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      26     507 
   Size:         26     481
 
EaeI y'GGCC_r

Cuts at:      0      98     507 
   Size:         98     409
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     191     507 
   Size:        101      90     316

  Fragments arranged by size: 

                 316     101      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     473     507 
   Size:        448      25      34

  Fragments arranged by size: 

                 448      34      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0     411     507 
   Size:        411      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     446     478     507 
   Size:        446      32      29

  Fragments arranged by size: 

                 446      32      29
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     420     507 
   Size:        420      87
 
MslI CAynn'nnrTG

Cuts at:      0     196     507 
   Size:        196     311
 
NcoI C'CATG_G

Cuts at:      0     191     507 
   Size:        191     316
 
NspI r_CATG'y

Cuts at:      0     261     507 
   Size:        261     246
 
PasI CC'CwG_GG

Cuts at:      0     277     507 
   Size:        277     230
 
PpuMI rG'GwC_Cy

Cuts at:      0     411     507 
   Size:        411      96
 
SfcI C'TryA_G

Cuts at:      0     174     507 
   Size:        174     333
 
StuI AGG'CCT

Cuts at:      0     425     507 
   Size:        425      82
 
StyI C'CwwG_G

Cuts at:      0     191     414     507 
   Size:        191     223      93

  Fragments arranged by size: 

                 223     191      93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     347     379     507 
   Size:        347      32     128

  Fragments arranged by size: 

                 347     128      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     108     217     507 
   Size:        108     109     290

  Fragments arranged by size: 

                 290     109     108
 

 Enzymes that do cut:

     AcuI   AflIII    AvrII    BamHI    BanII     BbsI    BbvCI     BglI
     BpmI   Bpu10I     BsaI    BsaBI    BsaXI    BseYI     BsgI Bsp1286I
    BsrBI    BsrDI    BstYI     BtgI    BtgZI     DrdI     EaeI     EarI
  Eco57MI EcoO109I    Hin4I     MmeI     MslI     NcoI     NspI     PasI
    PpuMI     SfcI     StuI     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaHI    BsaWI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI