(Linear) (Six-base) MAPSORT of: KIEE5171.seq  Check: 5108  from: 1  to: 483



KIEE5171.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     156     483 
   Size:        156     327
 
ApaLI G'TGCA_C

Cuts at:      0     422     483 
   Size:        422      61
 
BamHI G'GATC_C

Cuts at:      0     476     483 
   Size:        476       7
 
BanII G_rGCy'C

Cuts at:      0     113     368     483 
   Size:        113     255     115

  Fragments arranged by size: 

                 255     115     113
 
BlpI GC'TnA_GC

Cuts at:      0     283     483 
   Size:        283     200
 
Bme1580I G_kGCm'C

Cuts at:      0     426     483 
   Size:        426      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     381     483 
   Size:        381     102
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     399     483 
   Size:        399      84
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64     469     483 
   Size:         64     405      14

  Fragments arranged by size: 

                 405      64      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     426     483 
   Size:        426      57
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     332     483 
   Size:        332     151
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     368     426     483 
   Size:        113     255      58      57

  Fragments arranged by size: 

                 255     113      58      57
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     381     483 
   Size:        381     102
 
BsrBI CCG'CTC

Cuts at:      0      54     136     483 
   Size:         54      82     347

  Fragments arranged by size: 

                 347      82      54
 
BsrGI T'GTAC_A

Cuts at:      0     465     483 
   Size:        465      18
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     156     483 
   Size:        156     327
 
BstYI r'GATC_y

Cuts at:      0     434     476     483 
   Size:        434      42       7

  Fragments arranged by size: 

                 434      42       7
 
DraIII CAC_nnn'GTG

Cuts at:      0     351     483 
   Size:        351     132
 
EaeI y'GGCC_r

Cuts at:      0     356     483 
   Size:        356     127
 
EarI CTCTTCn'nnn_

Cuts at:      0     168     483 
   Size:        168     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     381     483 
   Size:        381     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0     153     483 
   Size:        153     330
 
MscI TGG'CCA

Cuts at:      0     358     483 
   Size:        358     125
 
MslI CAynn'nnrTG

Cuts at:      0     199     483 
   Size:        199     284
 
MspA1I CmG'CkG

Cuts at:      0     321     483 
   Size:        321     162
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     354     483 
   Size:        354     129
 
PfoI T'CCnGG_A

Cuts at:      0     397     483 
   Size:        397      86
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     483 
   Size:         16      32     435

  Fragments arranged by size: 

                 435      32      16
 
PstI C_TGCA'G

Cuts at:      0     213     483 
   Size:        213     270
 
SfcI C'TryA_G

Cuts at:      0      12     209     417     483 
   Size:         12     197     208      66

  Fragments arranged by size: 

                 208     197      66      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      59     483 
   Size:         59     424
 
TatI w'GTAC_w

Cuts at:      0     239     260     465     483 
   Size:        239      21     205      18

  Fragments arranged by size: 

                 239     205      21      18
 

 Enzymes that do cut:

    AlwNI    ApaLI    BamHI    BanII     BlpI Bme1580I     BpmI     BsaI
    BseRI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrGI   BstAPI
    BstYI   DraIII     EaeI     EarI  Eco57MI EcoO109I     MscI     MslI
   MspA1I    PflMI     PfoI     PpiI     PstI     SfcI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI