Restriction map for
pFN21AE5170
(Linear) (Six-base) MAPSORT of: KIEE5170.seq Check: 190 from: 1 to: 486
KIEE5170.orf
With 182 enzymes: SgfI *
January 29, 2014 11:49 ..
AccI GT'mk_AC
Cuts at: 0 337 486
Size: 337 149
AflII C'TTAA_G
Cuts at: 0 466 486
Size: 466 20
AflIII A'CryG_T
Cuts at: 0 213 486
Size: 213 273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 486
Size: 37 449
ApoI r'AATT_y
Cuts at: 0 381 486
Size: 381 105
AvaI C'yCGr_G
Cuts at: 0 250 486
Size: 250 236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 170 486
Size: 136 34 316
Fragments arranged by size:
316 136 34
Bme1580I G_kGCm'C
Cuts at: 0 332 486
Size: 332 154
BmgBI CAC'GTC
Cuts at: 0 198 486
Size: 198 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 397 409 486
Size: 325 72 12 77
Fragments arranged by size:
325 77 72 12
BsaHI Gr'CG_yC
Cuts at: 0 60 486
Size: 60 426
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 486
Size: 175 30 281
Fragments arranged by size:
281 175 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 268 486
Size: 151 117 218
Fragments arranged by size:
218 151 117
BseYI C'CCAG_C
Cuts at: 0 141 312 486
Size: 141 171 174
Fragments arranged by size:
174 171 141
BsiEI CG_ry'CG
Cuts at: 0 12 486
Size: 12 474
Bsp1286I G_dGCh'C
Cuts at: 0 332 486
Size: 332 154
BsrFI r'CCGG_y
Cuts at: 0 274 486
Size: 274 212
BstZ17I GTA'TAC
Cuts at: 0 338 486
Size: 338 148
BtgI C'CryG_G
Cuts at: 0 297 486
Size: 297 189
EaeI y'GGCC_r
Cuts at: 0 9 486
Size: 9 477
EagI C'GGCC_G
Cuts at: 0 9 486
Size: 9 477
EarI CTCTTCn'nnn_
Cuts at: 0 10 486
Size: 10 476
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 92 486
Size: 92 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 397 409 486
Size: 325 72 12 77
Fragments arranged by size:
325 77 72 12
EcoRI G'AATT_C
Cuts at: 0 381 486
Size: 381 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 462 486
Size: 462 24
MluI A'CGCG_T
Cuts at: 0 213 486
Size: 213 273
MslI CAynn'nnrTG
Cuts at: 0 37 230 486
Size: 37 193 256
Fragments arranged by size:
256 193 37
MspA1I CmG'CkG
Cuts at: 0 21 486
Size: 21 465
NaeI GCC'GGC
Cuts at: 0 276 486
Size: 276 210
NgoMIV G'CCGG_C
Cuts at: 0 274 486
Size: 274 212
PflMI CCAn_nnn'nTGG
Cuts at: 0 303 432 486
Size: 303 129 54
Fragments arranged by size:
303 129 54
PfoI T'CCnGG_A
Cuts at: 0 374 425 486
Size: 374 51 61
Fragments arranged by size:
374 61 51
PspXI vC'TCGA_Gb
Cuts at: 0 250 486
Size: 250 236
PstI C_TGCA'G
Cuts at: 0 446 486
Size: 446 40
SapI GCTCTTCn'nnn_
Cuts at: 0 10 486
Size: 10 476
SfcI C'TryA_G
Cuts at: 0 442 486
Size: 442 44
SmlI C'TyrA_G
Cuts at: 0 250 466 486
Size: 250 216 20
Fragments arranged by size:
250 216 20
StuI AGG'CCT
Cuts at: 0 362 464 486
Size: 362 102 22
Fragments arranged by size:
362 102 22
StyI C'CwwG_G
Cuts at: 0 290 486
Size: 290 196
XhoI C'TCGA_G
Cuts at: 0 250 486
Size: 250 236
Enzymes that do cut:
AccI AflII AflIII AlfI ApoI AvaI BcgI Bme1580I
BmgBI BpmI BsaHI BsaXI BseRI BseYI BsiEI Bsp1286I
BsrFI BstZ17I BtgI EaeI EagI EarI EciI Eco57MI
EcoRI Hin4I MluI MslI MspA1I NaeI NgoMIV PflMI
PfoI PspXI PstI SapI SfcI SmlI StuI StyI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MmeI MscI NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI