(Linear) (Six-base) MAPSORT of: KIEE5166.seq  Check: 1620  from: 1  to: 471



KIEE5166.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AccI GT'mk_AC

Cuts at:      0      86     471 
   Size:         86     385
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     381     471 
   Size:        132     249      90

  Fragments arranged by size: 

                 249     132      90
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      71     337     471 
   Size:         71     266     134

  Fragments arranged by size: 

                 266     134      71
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     338     372     471 
   Size:        338      34      99

  Fragments arranged by size: 

                 338      99      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      19     471 
   Size:         19     452
 
BlpI GC'TnA_GC

Cuts at:      0     410     471 
   Size:        410      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     244     471 
   Size:        244     227
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     444     471 
   Size:         58     386      27

  Fragments arranged by size: 

                 386      58      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     362     471 
   Size:        362     109
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     462     471 
   Size:        462       9
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     345     471 
   Size:        345     126
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     291     471 
   Size:        291     180
 
BstEII G'GTnAC_C

Cuts at:      0     315     471 
   Size:        315     156
 
EaeI y'GGCC_r

Cuts at:      0     153     471 
   Size:        153     318
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     444     471 
   Size:         58     386      27

  Fragments arranged by size: 

                 386      58      27
 
EcoRV GAT'ATC

Cuts at:      0     177     471 
   Size:        177     294
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      33      65     471 
   Size:         33      32     406

  Fragments arranged by size: 

                 406      33      32
 
MspA1I CmG'CkG

Cuts at:      0     129     381     471 
   Size:        129     252      90

  Fragments arranged by size: 

                 252     129      90
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     132     471 
   Size:        132     339
 
PfoI T'CCnGG_A

Cuts at:      0      53     471 
   Size:         53     418
 
PvuII CAG'CTG

Cuts at:      0     129     381     471 
   Size:        129     252      90

  Fragments arranged by size: 

                 252     129      90
 
SspI AAT'ATT

Cuts at:      0     186     471 
   Size:        186     285
 
TatI w'GTAC_w

Cuts at:      0     269     471 
   Size:        269     202
 
XmnI GAAnn'nnTTC

Cuts at:      0     227     471 
   Size:        227     244
 

 Enzymes that do cut:

     AccI    AlwNI     BbsI     BcgI    BciVI     BlpI     BmrI     BpmI
   Bpu10I     BsaI    BseRI    BspMI   BstEII     EaeI  Eco57MI    EcoRV
    Hin4I   MspA1I    PflMI     PfoI    PvuII     SspI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI