(Linear) (Six-base) MAPSORT of: KIEE5163.seq  Check: 5481  from: 1  to: 432



KIEE5163.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AclI AA'CG_TT

Cuts at:      0     347     432 
   Size:        347      85
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     432 
   Size:        231     201
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     432 
   Size:        146     286
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     354     388     432 
   Size:        354      34      44

  Fragments arranged by size: 

                 354      44      34
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     432 
   Size:        112     320
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     417     432 
   Size:        417      15
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     267     432 
   Size:        267     165
 
BtsI GCAGTG_nn'

Cuts at:      0     278     432 
   Size:        278     154
 
EaeI y'GGCC_r

Cuts at:      0     356     432 
   Size:        356      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     104     432 
   Size:        104     328
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     432 
   Size:        231     201
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       5     330     432 
   Size:          5     325     102

  Fragments arranged by size: 

                 325     102       5
 
MslI CAynn'nnrTG

Cuts at:      0     409     432 
   Size:        409      23
 
NspI r_CATG'y

Cuts at:      0      90     270     432 
   Size:         90     180     162

  Fragments arranged by size: 

                 180     162      90
 
PstI C_TGCA'G

Cuts at:      0     336     432 
   Size:        336      96
 
SfcI C'TryA_G

Cuts at:      0     332     432 
   Size:        332     100
 
SphI G_CATG'C

Cuts at:      0      90     432 
   Size:         90     342
 

 Enzymes that do cut:

     AclI     AcuI     BbsI     BcgI    BseRI    BsmBI   BstAPI     BtsI
     EaeI     EarI  Eco57MI     MmeI     MslI     NspI     PstI     SfcI
     SphI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI