(Linear) (Six-base) MAPSORT of: KIEE5161.seq  Check: 6002  from: 1  to: 402



KIEE5161.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     162     402 
   Size:        162     240
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     163     402 
   Size:        163     239
 
AvaI C'yCGr_G

Cuts at:      0      23     236     402 
   Size:         23     213     166

  Fragments arranged by size: 

                 213     166      23
 
BamHI G'GATC_C

Cuts at:      0       3     402 
   Size:          3     399
 
BanI G'GyrC_C

Cuts at:      0     266     297     402 
   Size:        266      31     105

  Fragments arranged by size: 

                 266     105      31
 
BanII G_rGCy'C

Cuts at:      0     197     211     402 
   Size:        197      14     191

  Fragments arranged by size: 

                 197     191      14
 
BbvCI CC'TCA_GC

Cuts at:      0     391     402 
   Size:        391      11
 
Bme1580I G_kGCm'C

Cuts at:      0     269     300     402 
   Size:        269      31     102

  Fragments arranged by size: 

                 269     102      31
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     211     351     402 
   Size:        184      27     140      51

  Fragments arranged by size: 

                 184     140      51      27
 
Bpu10I CC'TnA_GC

Cuts at:      0     391     402 
   Size:        391      11
 
BsaWI w'CCGG_w

Cuts at:      0     353     402 
   Size:        353      49
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     354     402 
   Size:        354      48
 
BseYI C'CCAG_C

Cuts at:      0      84     402 
   Size:         84     318
 
Bsp1286I G_dGCh'C

Cuts at:      0     197     211     269     300     402 
   Size:        197      14      58      31     102

  Fragments arranged by size: 

                 197     102      58      31      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     162     402 
   Size:        162     240
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     375     402 
   Size:        375      27
 
BstYI r'GATC_y

Cuts at:      0       3     402 
   Size:          3     399
 
BtgI C'CryG_G

Cuts at:      0     261     402 
   Size:        261     141
 
EarI CTCTTCn'nnn_

Cuts at:      0      49     402 
   Size:         49     353
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     211     351     402 
   Size:        184      27     140      51

  Fragments arranged by size: 

                 184     140      51      27
 
HaeII r_GCGC'y

Cuts at:      0      31     402 
   Size:         31     371
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     169     198     201     230     402 
   Size:        169      29       3      29     172

  Fragments arranged by size: 

                 172     169      29      29       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     402 
   Size:        143     259
 
MspA1I CmG'CkG

Cuts at:      0      84     402 
   Size:         84     318
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      84     402 
   Size:         84     318
 
PfoI T'CCnGG_A

Cuts at:      0     200     402 
   Size:        200     202
 
PstI C_TGCA'G

Cuts at:      0     173     402 
   Size:        173     229
 
SapI GCTCTTCn'nnn_

Cuts at:      0      49     402 
   Size:         49     353
 
SfcI C'TryA_G

Cuts at:      0     169     402 
   Size:        169     233
 
SmaI CCC'GGG

Cuts at:      0      25     238     402 
   Size:         25     213     164

  Fragments arranged by size: 

                 213     164      25
 
XmaI C'CCGG_G

Cuts at:      0      23     236     402 
   Size:         23     213     166

  Fragments arranged by size: 

                 213     166      23
 

 Enzymes that do cut:

     AarI     AlfI     AvaI    BamHI     BanI    BanII    BbvCI Bme1580I
     BpmI   Bpu10I    BsaWI    BseRI    BseYI Bsp1286I    BspMI    BstXI
    BstYI     BtgI     EarI  Eco57MI    HaeII    Hin4I     MmeI   MspA1I
    PflMI     PfoI     PstI     SapI     SfcI     SmaI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI