(Linear) (Six-base) MAPSORT of: KIEE5157.seq  Check: 952  from: 1  to: 378



KIEE5157.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     325     378 
   Size:        195     130      53

  Fragments arranged by size: 

                 195     130      53
 
AfeI AGC'GCT

Cuts at:      0      36     378 
   Size:         36     342
 
AflII C'TTAA_G

Cuts at:      0     235     378 
   Size:        235     143
 
AvaI C'yCGr_G

Cuts at:      0     116     378 
   Size:        116     262
 
BamHI G'GATC_C

Cuts at:      0     273     378 
   Size:        273     105
 
BglI GCCn_nnn'nGGC

Cuts at:      0     296     378 
   Size:        296      82
 
BlpI GC'TnA_GC

Cuts at:      0      38     378 
   Size:         38     340
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     378 
   Size:         51     327
 
BsaHI Gr'CG_yC

Cuts at:      0       4     378 
   Size:          4     374
 
BsmI GAATG_Cn'

Cuts at:      0      99     378 
   Size:         99     279
 
BsrFI r'CCGG_y

Cuts at:      0      21     378 
   Size:         21     357
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     159     378 
   Size:        159     219
 
BstYI r'GATC_y

Cuts at:      0     179     273     378 
   Size:        179      94     105

  Fragments arranged by size: 

                 179     105      94
 
BtgI C'CryG_G

Cuts at:      0     167     342     378 
   Size:        167     175      36

  Fragments arranged by size: 

                 175     167      36
 
BtsI GCAGTG_nn'

Cuts at:      0     104     378 
   Size:        104     274
 
EaeI y'GGCC_r

Cuts at:      0     170     368     378 
   Size:        170     198      10

  Fragments arranged by size: 

                 198     170      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      72     378 
   Size:         72     306
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     325     378 
   Size:        195     130      53

  Fragments arranged by size: 

                 195     130      53
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      61     378 
   Size:         61     317
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     250     378 
   Size:        250     128
 
FspI TGC'GCA

Cuts at:      0     103     378 
   Size:        103     275
 
FspAI rTGC'GCAy

Cuts at:      0     103     378 
   Size:        103     275
 
HaeII r_GCGC'y

Cuts at:      0      38     313     378 
   Size:         38     275      65

  Fragments arranged by size: 

                 275      65      38
 
MscI TGG'CCA

Cuts at:      0     172     378 
   Size:        172     206
 
MspA1I CmG'CkG

Cuts at:      0     215     378 
   Size:        215     163
 
NcoI C'CATG_G

Cuts at:      0     342     378 
   Size:        342      36
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     343     378 
   Size:        343      35
 
PvuII CAG'CTG

Cuts at:      0     215     378 
   Size:        215     163
 
SmlI C'TyrA_G

Cuts at:      0      30     235     378 
   Size:         30     205     143

  Fragments arranged by size: 

                 205     143      30
 
StyI C'CwwG_G

Cuts at:      0     342     378 
   Size:        342      36
 

 Enzymes that do cut:

     AcuI     AfeI    AflII     AvaI    BamHI     BglI     BlpI    BpuEI
    BsaHI     BsmI    BsrFI   BstAPI    BstYI     BtgI     BtsI     EaeI
     EciI  Eco57MI    EcoNI     FalI     FspI    FspAI    HaeII     MscI
   MspA1I     NcoI    PflMI    PvuII     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FseI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI