(Linear) (Six-base) MAPSORT of: KIEE5154.seq  Check: 7379  from: 1  to: 300



KIEE5154.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      81     300 
   Size:         81     219
 
Acc65I G'GTAC_C

Cuts at:      0     104     300 
   Size:        104     196
 
BanI G'GyrC_C

Cuts at:      0     104     264     300 
   Size:        104     160      36

  Fragments arranged by size: 

                 160     104      36
 
BanII G_rGCy'C

Cuts at:      0     278     300 
   Size:        278      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     103     300 
   Size:        103     197
 
BglI GCCn_nnn'nGGC

Cuts at:      0     180     300 
   Size:        180     120
 
BglII A'GATC_T

Cuts at:      0      57     300 
   Size:         57     243
 
BmgBI CAC'GTC

Cuts at:      0     101     300 
   Size:        101     199
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     178     300 
   Size:         28     150     122

  Fragments arranged by size: 

                 150     122      28
 
Bpu10I CC'TnA_GC

Cuts at:      0      79     300 
   Size:         79     221
 
BseYI C'CCAG_C

Cuts at:      0     277     300 
   Size:        277      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     278     300 
   Size:        278      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      81     300 
   Size:         81     219
 
BsrFI r'CCGG_y

Cuts at:      0     255     300 
   Size:        255      45
 
BssHII G'CGCG_C

Cuts at:      0     234     300 
   Size:        234      66
 
BstYI r'GATC_y

Cuts at:      0      57     300 
   Size:         57     243
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     274     300 
   Size:        274      26
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     178     300 
   Size:         28     150     122

  Fragments arranged by size: 

                 150     122      28
 
KpnI G_GTAC'C

Cuts at:      0     108     300 
   Size:        108     192
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     300 
   Size:        229      71
 
PasI CC'CwG_GG

Cuts at:      0      72     300 
   Size:         72     228
 
PstI C_TGCA'G

Cuts at:      0     146     300 
   Size:        146     154
 
SfcI C'TryA_G

Cuts at:      0     142     300 
   Size:        142     158
 
StyI C'CwwG_G

Cuts at:      0     176     239     300 
   Size:        176      63      61

  Fragments arranged by size: 

                 176      63      61
 
XmnI GAAnn'nnTTC

Cuts at:      0      16     300 
   Size:         16     284
 

 Enzymes that do cut:

     AarI   Acc65I     BanI    BanII     BbsI     BglI    BglII    BmgBI
     BpmI   Bpu10I    BseYI Bsp1286I    BspMI    BsrFI   BssHII    BstYI
     EciI  Eco57MI     KpnI     MmeI     PasI     PstI     SfcI     StyI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI