(Linear) (Six-base) MAPSORT of: KIEE5150.seq  Check: 8244  from: 1  to: 258



KIEE5150.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AflII C'TTAA_G

Cuts at:      0      67     258 
   Size:         67     191
 
EarI CTCTTCn'nnn_

Cuts at:      0     224     258 
   Size:        224      34
 
MslI CAynn'nnrTG

Cuts at:      0     121     176     258 
   Size:        121      55      82

  Fragments arranged by size: 

                 121      82      55
 
NspI r_CATG'y

Cuts at:      0     126     258 
   Size:        126     132
 
SfcI C'TryA_G

Cuts at:      0     104     258 
   Size:        104     154
 
SmlI C'TyrA_G

Cuts at:      0      67     258 
   Size:         67     191
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     166     258 
   Size:        166      92
 
TatI w'GTAC_w

Cuts at:      0      83     258 
   Size:         83     175
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      38      70     258 
   Size:         38      32     188

  Fragments arranged by size: 

                 188      38      32
 

 Enzymes that do cut:

    AflII     EarI     MslI     NspI     SfcI     SmlI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI