(Linear) (Six-base) MAPSORT of: KIEE5149.seq  Check: 455  from: 1  to: 276



KIEE5149.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     193     276 
   Size:        193      83
 
BanI G'GyrC_C

Cuts at:      0     199     276 
   Size:        199      77
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     190     258     276 
   Size:        190      68      18

  Fragments arranged by size: 

                 190      68      18
 
BsaWI w'CCGG_w

Cuts at:      0      65     276 
   Size:         65     211
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     138     276 
   Size:        138     138
 
BspEI T'CCGG_A

Cuts at:      0      65     276 
   Size:         65     211
 
BstYI r'GATC_y

Cuts at:      0     158     276 
   Size:        158     118
 
EaeI y'GGCC_r

Cuts at:      0       2     276 
   Size:          2     274
 
EarI CTCTTCn'nnn_

Cuts at:      0     136     276 
   Size:        136     140
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     231     276 
   Size:        231      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0      44      56     276 
   Size:         44      12     220

  Fragments arranged by size: 

                 220      44      12
 
MscI TGG'CCA

Cuts at:      0       4     276 
   Size:          4     272
 
MspA1I CmG'CkG

Cuts at:      0     226     276 
   Size:        226      50
 
PpuMI rG'GwC_Cy

Cuts at:      0      44     276 
   Size:         44     232
 
SanDI GG'GwC_CC

Cuts at:      0      44     276 
   Size:         44     232
 
TatI w'GTAC_w

Cuts at:      0     107     276 
   Size:        107     169
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     157     189     276 
   Size:        157      32      87

  Fragments arranged by size: 

                 157      87      32
 

 Enzymes that do cut:

    AlwNI     BanI     BbsI    BsaWI    BsmBI    BspEI    BstYI     EaeI
     EarI     EciI EcoO109I     MscI   MspA1I    PpuMI    SanDI     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI