(Linear) (Six-base) MAPSORT of: KIEE5145.seq  Check: 9415  from: 1  to: 300



KIEE5145.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      52     300 
   Size:         52     248
 
BamHI G'GATC_C

Cuts at:      0     192     300 
   Size:        192     108
 
BanII G_rGCy'C

Cuts at:      0       9     177     206     227     300 
   Size:          9     168      29      21      73

  Fragments arranged by size: 

                 168      73      29      21       9
 
BglI GCCn_nnn'nGGC

Cuts at:      0      28     300 
   Size:         28     272
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     172     300 
   Size:        172     128
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     300 
   Size:        220      80
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     135     300 
   Size:        135     165
 
BsaWI w'CCGG_w

Cuts at:      0     231     300 
   Size:        231      69
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     164     194     300 
   Size:        164      30     106

  Fragments arranged by size: 

                 164     106      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7     300 
   Size:          7     293
 
BseYI C'CCAG_C

Cuts at:      0      73     168     300 
   Size:         73      95     132

  Fragments arranged by size: 

                 132      95      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     119     300 
   Size:        101      18     181

  Fragments arranged by size: 

                 181     101      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     300 
   Size:        227      73
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     177     206     227     300 
   Size:          9     168      29      21      73

  Fragments arranged by size: 

                 168      73      29      21       9
 
BspEI T'CCGG_A

Cuts at:      0     231     300 
   Size:        231      69
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      52     300 
   Size:         52     248
 
BstYI r'GATC_y

Cuts at:      0     192     300 
   Size:        192     108
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      33     300 
   Size:         33     267
 
EaeI y'GGCC_r

Cuts at:      0      35      54     300 
   Size:         35      19     246

  Fragments arranged by size: 

                 246      35      19
 
EarI CTCTTCn'nnn_

Cuts at:      0     133     300 
   Size:        133     167
 
EcoICRI GAG'CTC

Cuts at:      0     225     300 
   Size:        225      75
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     300 
   Size:        220      80
 
HaeII r_GCGC'y

Cuts at:      0     153     285     300 
   Size:        153     132      15

  Fragments arranged by size: 

                 153     132      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     164     196     300 
   Size:        164      32     104

  Fragments arranged by size: 

                 164     104      32
 
MscI TGG'CCA

Cuts at:      0      56     300 
   Size:         56     244
 
MspA1I CmG'CkG

Cuts at:      0      77     300 
   Size:         77     223
 
SacI G_AGCT'C

Cuts at:      0     227     300 
   Size:        227      73
 
StuI AGG'CCT

Cuts at:      0     274     300 
   Size:        274      26
 

 Enzymes that do cut:

     AarI    BamHI    BanII     BglI     BmrI     BpmI     BsaI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI
    BstYI    BtgZI     EaeI     EarI  EcoICRI  Eco57MI    HaeII    Hin4I
     MscI   MspA1I     SacI     StuI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI