(Linear) (Six-base) MAPSORT of: KIEE5144.seq  Check: 2422  from: 1  to: 276



KIEE5144.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

ApoI r'AATT_y

Cuts at:      0     117     276 
   Size:        117     159
 
BanII G_rGCy'C

Cuts at:      0     234     276 
   Size:        234      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     269     276 
   Size:        269       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     116     276 
   Size:         48      68     160

  Fragments arranged by size: 

                 160      68      48
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     212     276 
   Size:        212      64
 
BsiHKAI G_wGCw'C

Cuts at:      0     234     276 
   Size:        234      42
 
BsmI GAATG_Cn'

Cuts at:      0     190     276 
   Size:        190      86
 
Bsp1286I G_dGCh'C

Cuts at:      0     234     276 
   Size:        234      42
 
BstBI TT'CG_AA

Cuts at:      0     182     276 
   Size:        182      94
 
EarI CTCTTCn'nnn_

Cuts at:      0     245     276 
   Size:        245      31
 
EcoICRI GAG'CTC

Cuts at:      0     232     276 
   Size:        232      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     116     276 
   Size:         48      68     160

  Fragments arranged by size: 

                 160      68      48
 
HincII GTy'rAC

Cuts at:      0     160     276 
   Size:        160     116
 
SacI G_AGCT'C

Cuts at:      0     234     276 
   Size:        234      42
 

 Enzymes that do cut:

     ApoI    BanII     BmrI     BpmI     BsaI  BsiHKAI     BsmI Bsp1286I
    BstBI     EarI  EcoICRI  Eco57MI   HincII     SacI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI