(Linear) (Six-base) MAPSORT of: KIEE5141.seq  Check: 4542  from: 1  to: 327



KIEE5141.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     327 
   Size:        188     139
 
ApoI r'AATT_y

Cuts at:      0      74     327 
   Size:         74     253
 
BbvCI CC'TCA_GC

Cuts at:      0       9     327 
   Size:          9     318
 
Bpu10I CC'TnA_GC

Cuts at:      0       9     327 
   Size:          9     318
 
BsaHI Gr'CG_yC

Cuts at:      0      91     327 
   Size:         91     236
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     248     278     327 
   Size:        248      30      49

  Fragments arranged by size: 

                 248      49      30
 
BsmI GAATG_Cn'

Cuts at:      0     228     327 
   Size:        228      99
 
BsrFI r'CCGG_y

Cuts at:      0      54     327 
   Size:         54     273
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      90     327 
   Size:         90     237
 
EaeI y'GGCC_r

Cuts at:      0     126     327 
   Size:        126     201
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     327 
   Size:        188     139
 
EcoRI G'AATT_C

Cuts at:      0      74     327 
   Size:         74     253
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      95     232     327 
   Size:         95     137      95

  Fragments arranged by size: 

                 137      95      95
 
HaeII r_GCGC'y

Cuts at:      0      34     327 
   Size:         34     293
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     248     280     327 
   Size:        248      32      47

  Fragments arranged by size: 

                 248      47      32
 
MspA1I CmG'CkG

Cuts at:      0     102     327 
   Size:        102     225
 
PvuII CAG'CTG

Cuts at:      0     102     327 
   Size:        102     225
 
StyI C'CwwG_G

Cuts at:      0      79     327 
   Size:         79     248
 
XmnI GAAnn'nnTTC

Cuts at:      0     215     327 
   Size:        215     112
 

 Enzymes that do cut:

     AcuI     ApoI    BbvCI   Bpu10I    BsaHI    BsaXI     BsmI    BsrFI
     DrdI     EaeI  Eco57MI    EcoRI     FalI    HaeII    Hin4I   MspA1I
    PvuII     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI