(Linear) (Six-base) MAPSORT of: KIEE5140.seq  Check: 4929  from: 1  to: 348



KIEE5140.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     348 
   Size:        133     215
 
BanI G'GyrC_C

Cuts at:      0     136     348 
   Size:        136     212
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     211     348 
   Size:        211     137
 
Bme1580I G_kGCm'C

Cuts at:      0     141     348 
   Size:        141     207
 
BseYI C'CCAG_C

Cuts at:      0     128     348 
   Size:        128     220
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     348 
   Size:        103     245
 
BsmI GAATG_Cn'

Cuts at:      0     103     348 
   Size:        103     245
 
Bsp1286I G_dGCh'C

Cuts at:      0     141     348 
   Size:        141     207
 
BsrBI CCG'CTC

Cuts at:      0      73     348 
   Size:         73     275
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     161     348 
   Size:        161     187
 
BtsI GCAGTG_nn'

Cuts at:      0     318     348 
   Size:        318      30
 
DraIII CAC_nnn'GTG

Cuts at:      0     314     348 
   Size:        314      34
 
EarI CTCTTCn'nnn_

Cuts at:      0     127     348 
   Size:        127     221
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     133     348 
   Size:        133     215
 
MslI CAynn'nnrTG

Cuts at:      0     280     348 
   Size:        280      68
 
NspI r_CATG'y

Cuts at:      0      61     348 
   Size:         61     287
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249     348 
   Size:        217      32      99

  Fragments arranged by size: 

                 217      99      32
 
PstI C_TGCA'G

Cuts at:      0     325     348 
   Size:        325      23
 
ScaI AGT'ACT

Cuts at:      0     327     348 
   Size:        327      21
 
SfcI C'TryA_G

Cuts at:      0       9     106     321     348 
   Size:          9      97     215      27

  Fragments arranged by size: 

                 215      97      27       9
 
TatI w'GTAC_w

Cuts at:      0     325     348 
   Size:        325      23
 
XbaI T'CTAG_A

Cuts at:      0     172     348 
   Size:        172     176
 

 Enzymes that do cut:

     AcuI     BanI     BbsI Bme1580I    BseYI     BsgI     BsmI Bsp1286I
    BsrBI   BstAPI     BtsI   DraIII     EarI  Eco57MI     MslI     NspI
     PpiI     PstI     ScaI     SfcI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI