(Linear) (Six-base) MAPSORT of: KIEE5137.seq  Check: 1822  from: 1  to: 438



KIEE5137.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     438 
   Size:         49     389
 
AlwNI CAG_nnn'CTG

Cuts at:      0      51      54     393     438 
   Size:         51       3     339      45

  Fragments arranged by size: 

                 339      51      45       3
 
BanI G'GyrC_C

Cuts at:      0     328     438 
   Size:        328     110
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     436     438 
   Size:        436       2
 
BsaAI yAC'GTr

Cuts at:      0     368     438 
   Size:        368      70
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     393     438 
   Size:        393      45
 
BseYI C'CCAG_C

Cuts at:      0     183     438 
   Size:        183     255
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     438 
   Size:          6     432
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     228     438 
   Size:        228     210
 
BspHI T'CATG_A

Cuts at:      0     314     377     438 
   Size:        314      63      61

  Fragments arranged by size: 

                 314      63      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     431     438 
   Size:        431       7
 
BsrGI T'GTAC_A

Cuts at:      0     344     438 
   Size:        344      94
 
Bsu36I CC'TnA_GG

Cuts at:      0     416     438 
   Size:        416      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     438 
   Size:         49     389
 
EcoO109I rG'GnC_Cy

Cuts at:      0     299     420     438 
   Size:        299     121      18

  Fragments arranged by size: 

                 299     121      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     271     303     438 
   Size:        271      32     135

  Fragments arranged by size: 

                 271     135      32
 
MfeI C'AATT_G

Cuts at:      0     251     438 
   Size:        251     187
 
MspA1I CmG'CkG

Cuts at:      0      51     183     438 
   Size:         51     132     255

  Fragments arranged by size: 

                 255     132      51
 
NspI r_CATG'y

Cuts at:      0     273     438 
   Size:        273     165
 
PfoI T'CCnGG_A

Cuts at:      0     386     438 
   Size:        386      52
 
PpuMI rG'GwC_Cy

Cuts at:      0     420     438 
   Size:        420      18
 
PstI C_TGCA'G

Cuts at:      0     307     428     438 
   Size:        307     121      10

  Fragments arranged by size: 

                 307     121      10
 
PvuII CAG'CTG

Cuts at:      0      51     438 
   Size:         51     387
 
RsrII CG'GwC_CG

Cuts at:      0     203     438 
   Size:        203     235
 
SbfI CC_TGCA'GG

Cuts at:      0     307     438 
   Size:        307     131
 
SfcI C'TryA_G

Cuts at:      0      96     303     424     438 
   Size:         96     207     121      14

  Fragments arranged by size: 

                 207     121      96      14
 
SnaBI TAC'GTA

Cuts at:      0     368     438 
   Size:        368      70
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     145     438 
   Size:        145     293
 
TatI w'GTAC_w

Cuts at:      0     344     438 
   Size:        344      94
 

 Enzymes that do cut:

     AcuI    AlwNI     BanI     BbsI    BsaAI    BseRI    BseYI     BsgI
    BsmBI    BspHI    BspMI    BsrGI   Bsu36I  Eco57MI EcoO109I    Hin4I
     MfeI   MspA1I     NspI     PfoI    PpuMI     PstI    PvuII    RsrII
     SbfI     SfcI    SnaBI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI
    BsiWI     BsmI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI     SacI    SacII     SalI    SanDI     SapI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI