(Linear) (Six-base) MAPSORT of: KIEE5135.seq  Check: 5409  from: 1  to: 324



KIEE5135.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AflIII A'CryG_T

Cuts at:      0     262     324 
   Size:        262      62
 
AhdI GACnn_n'nnGTC

Cuts at:      0     263     324 
   Size:        263      61
 
AvaI C'yCGr_G

Cuts at:      0     191     324 
   Size:        191     133
 
BamHI G'GATC_C

Cuts at:      0      92     324 
   Size:         92     232
 
BglI GCCn_nnn'nGGC

Cuts at:      0     194     324 
   Size:        194     130
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     166     324 
   Size:        150      16     158

  Fragments arranged by size: 

                 158     150      16
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     250     324 
   Size:        250      74
 
BsaWI w'CCGG_w

Cuts at:      0     295     324 
   Size:        295      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7      10      13     324 
   Size:          7       3       3     311

  Fragments arranged by size: 

                 311       7       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     324 
   Size:        198     126
 
BsiHKAI G_wGCw'C

Cuts at:      0      67     324 
   Size:         67     257
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     324 
   Size:         67     257
 
BsrFI r'CCGG_y

Cuts at:      0     106     208     324 
   Size:        106     102     116

  Fragments arranged by size: 

                 116     106     102
 
BssHII G'CGCG_C

Cuts at:      0     249     301     324 
   Size:        249      52      23

  Fragments arranged by size: 

                 249      52      23
 
BssSI C'ACGA_G

Cuts at:      0     237     324 
   Size:        237      87
 
BstYI r'GATC_y

Cuts at:      0      92     324 
   Size:         92     232
 
BtgI C'CryG_G

Cuts at:      0      68     324 
   Size:         68     256
 
BtsI GCAGTG_nn'

Cuts at:      0     267     324 
   Size:        267      57
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     216     324 
   Size:        216     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     166     324 
   Size:        150      16     158

  Fragments arranged by size: 

                 158     150      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174     324 
   Size:        174     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     324 
   Size:        249      32      43

  Fragments arranged by size: 

                 249      43      32
 
NaeI GCC'GGC

Cuts at:      0     108     324 
   Size:        108     216
 
NcoI C'CATG_G

Cuts at:      0      68     324 
   Size:         68     256
 
NgoMIV G'CCGG_C

Cuts at:      0     106     324 
   Size:        106     218
 
NspI r_CATG'y

Cuts at:      0     266     324 
   Size:        266      58
 
PciI A'CATG_T

Cuts at:      0     262     324 
   Size:        262      62
 
PfoI T'CCnGG_A

Cuts at:      0      95     324 
   Size:         95     229
 
PpuMI rG'GwC_Cy

Cuts at:      0     174     324 
   Size:        174     150
 
SspI AAT'ATT

Cuts at:      0     119     324 
   Size:        119     205
 
StyI C'CwwG_G

Cuts at:      0      68     324 
   Size:         68     256
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174     324 
   Size:        174     150
 

 Enzymes that do cut:

   AflIII     AhdI     AvaI    BamHI     BglI     BpmI     BsaI    BsaWI
    BseRI     BsgI  BsiHKAI Bsp1286I    BsrFI   BssHII    BssSI    BstYI
     BtgI     BtsI     EciI  Eco57MI EcoO109I    Hin4I     NaeI     NcoI
   NgoMIV     NspI     PciI     PfoI    PpuMI     SspI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI