(Linear) (Six-base) MAPSORT of: KIEE5134.seq  Check: 864  from: 1  to: 495



KIEE5134.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     495 
   Size:        341     154
 
AfeI AGC'GCT

Cuts at:      0     142     495 
   Size:        142     353
 
BlpI GC'TnA_GC

Cuts at:      0     377     495 
   Size:        377     118
 
Bme1580I G_kGCm'C

Cuts at:      0      62     495 
   Size:         62     433
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     333     423     495 
   Size:        333      90      72

  Fragments arranged by size: 

                 333      90      72
 
BsaAI yAC'GTr

Cuts at:      0     276     495 
   Size:        276     219
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     221     251     353     383     495 
   Size:        221      30     102      30     112

  Fragments arranged by size: 

                 221     112     102      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     481     484     495 
   Size:        412      69       3      11

  Fragments arranged by size: 

                 412      69      11       3
 
BseYI C'CCAG_C

Cuts at:      0     372     495 
   Size:        372     123
 
BsiHKAI G_wGCw'C

Cuts at:      0      53     263     495 
   Size:         53     210     232

  Fragments arranged by size: 

                 232     210      53
 
Bsp1286I G_dGCh'C

Cuts at:      0      53      62     263     495 
   Size:         53       9     201     232

  Fragments arranged by size: 

                 232     201      53       9
 
BspHI T'CATG_A

Cuts at:      0     456     495 
   Size:        456      39
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      57     495 
   Size:         57     438
 
Bsu36I CC'TnA_GG

Cuts at:      0     308     495 
   Size:        308     187
 
DraIII CAC_nnn'GTG

Cuts at:      0      57     495 
   Size:         57     438
 
EarI CTCTTCn'nnn_

Cuts at:      0     218     473     495 
   Size:        218     255      22

  Fragments arranged by size: 

                 255     218      22
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     495 
   Size:        341     154
 
EcoO109I rG'GnC_Cy

Cuts at:      0      95     495 
   Size:         95     400
 
HaeII r_GCGC'y

Cuts at:      0     144     495 
   Size:        144     351
 
HincII GTy'rAC

Cuts at:      0     213     435     495 
   Size:        213     222      60

  Fragments arranged by size: 

                 222     213      60
 
HpaI GTT'AAC

Cuts at:      0     435     495 
   Size:        435      60
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     495 
   Size:         89     406
 
MslI CAynn'nnrTG

Cuts at:      0     461     495 
   Size:        461      34
 
MspA1I CmG'CkG

Cuts at:      0     376     495 
   Size:        376     119
 
NspI r_CATG'y

Cuts at:      0      28     159     495 
   Size:         28     131     336

  Fragments arranged by size: 

                 336     131      28
 
PasI CC'CwG_GG

Cuts at:      0     100     495 
   Size:        100     395
 
PmlI CAC'GTG

Cuts at:      0     276     495 
   Size:        276     219
 
PpuMI rG'GwC_Cy

Cuts at:      0      95     495 
   Size:         95     400
 
PstI C_TGCA'G

Cuts at:      0     290     495 
   Size:        290     205
 
PvuII CAG'CTG

Cuts at:      0     376     495 
   Size:        376     119
 
SfcI C'TryA_G

Cuts at:      0     286     495 
   Size:        286     209
 
SphI G_CATG'C

Cuts at:      0     159     495 
   Size:        159     336
 
StuI AGG'CCT

Cuts at:      0     313     495 
   Size:        313     182
 
StyI C'CwwG_G

Cuts at:      0     132     495 
   Size:        132     363
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253     495 
   Size:        221      32     242

  Fragments arranged by size: 

                 242     221      32
 

 Enzymes that do cut:

     AcuI     AfeI     BlpI Bme1580I     BplI    BsaAI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspHI   BstAPI   Bsu36I   DraIII     EarI
  Eco57MI EcoO109I    HaeII   HincII     HpaI     MmeI     MslI   MspA1I
     NspI     PasI     PmlI    PpuMI     PstI    PvuII     SfcI     SphI
     StuI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI