Restriction map for
pFN21AE5130
(Linear) (Six-base) MAPSORT of: KIEE5130.seq Check: 4387 from: 1 to: 450
KIEE5130.orf
With 182 enzymes: SgfI *
January 29, 2014 11:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 450
Size: 379 71
ApaLI G'TGCA_C
Cuts at: 0 224 450
Size: 224 226
ApoI r'AATT_y
Cuts at: 0 314 450
Size: 314 136
AvaI C'yCGr_G
Cuts at: 0 18 450
Size: 18 432
BanI G'GyrC_C
Cuts at: 0 54 450
Size: 54 396
BanII G_rGCy'C
Cuts at: 0 43 164 248 450
Size: 43 121 84 202
Fragments arranged by size:
202 121 84 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 383 450
Size: 383 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 230 264 319 353 450
Size: 230 34 55 34 97
Fragments arranged by size:
230 97 55 34 34
BlpI GC'TnA_GC
Cuts at: 0 37 450
Size: 37 413
Bme1580I G_kGCm'C
Cuts at: 0 228 450
Size: 228 222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 129 450
Size: 129 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 450
Size: 124 326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 450
Size: 44 406
BsaI GGTCTCn'nnnn_
Cuts at: 0 258 450
Size: 258 192
BsaWI w'CCGG_w
Cuts at: 0 429 450
Size: 429 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 450
Size: 277 30 143
Fragments arranged by size:
277 143 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 97 214 370 450
Size: 94 3 117 156 80
Fragments arranged by size:
156 117 94 80 3
BsiEI CG_ry'CG
Cuts at: 0 234 450
Size: 234 216
BsiHKAI G_wGCw'C
Cuts at: 0 43 228 248 450
Size: 43 185 20 202
Fragments arranged by size:
202 185 43 20
Bsp1286I G_dGCh'C
Cuts at: 0 43 164 228 248 450
Size: 43 121 64 20 202
Fragments arranged by size:
202 121 64 43 20
BsrFI r'CCGG_y
Cuts at: 0 251 450
Size: 251 199
BstEII G'GTnAC_C
Cuts at: 0 87 204 438 450
Size: 87 117 234 12
Fragments arranged by size:
234 117 87 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 178 450
Size: 102 76 272
Fragments arranged by size:
272 102 76
BtsI GCAGTG_nn'
Cuts at: 0 357 450
Size: 357 93
DraIII CAC_nnn'GTG
Cuts at: 0 223 450
Size: 223 227
EaeI y'GGCC_r
Cuts at: 0 231 450
Size: 231 219
EagI C'GGCC_G
Cuts at: 0 231 450
Size: 231 219
EcoICRI GAG'CTC
Cuts at: 0 41 246 450
Size: 41 205 204
Fragments arranged by size:
205 204 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 379 450
Size: 124 255 71
Fragments arranged by size:
255 124 71
HaeII r_GCGC'y
Cuts at: 0 258 450
Size: 258 192
HincII GTy'rAC
Cuts at: 0 296 450
Size: 296 154
HpaI GTT'AAC
Cuts at: 0 296 450
Size: 296 154
MslI CAynn'nnrTG
Cuts at: 0 94 450
Size: 94 356
MspA1I CmG'CkG
Cuts at: 0 36 364 450
Size: 36 328 86
Fragments arranged by size:
328 86 36
NaeI GCC'GGC
Cuts at: 0 253 450
Size: 253 197
NgoMIV G'CCGG_C
Cuts at: 0 251 450
Size: 251 199
PfoI T'CCnGG_A
Cuts at: 0 101 450
Size: 101 349
PstI C_TGCA'G
Cuts at: 0 12 140 450
Size: 12 128 310
Fragments arranged by size:
310 128 12
SacI G_AGCT'C
Cuts at: 0 43 248 450
Size: 43 205 202
Fragments arranged by size:
205 202 43
SfcI C'TryA_G
Cuts at: 0 8 136 407 450
Size: 8 128 271 43
Fragments arranged by size:
271 128 43 8
SmlI C'TyrA_G
Cuts at: 0 59 450
Size: 59 391
StyI C'CwwG_G
Cuts at: 0 44 281 450
Size: 44 237 169
Fragments arranged by size:
237 169 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 108 450
Size: 108 342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 99 450
Size: 99 351
Enzymes that do cut:
AcuI ApaLI ApoI AvaI BanI BanII BbsI BcgI
BlpI Bme1580I BplI BpmI BpuEI BsaI BsaWI BsaXI
BseRI BsiEI BsiHKAI Bsp1286I BsrFI BstEII BstXI BtsI
DraIII EaeI EagI EcoICRI Eco57MI HaeII HincII HpaI
MslI MspA1I NaeI NgoMIV PfoI PstI SacI SfcI
SmlI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I
BsaAI BsaBI BsaHI BseYI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DrdI EarI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII KasI
KpnI MfeI MluI MmeI MscI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI