(Linear) (Six-base) MAPSORT of: KIEE5124.seq  Check: 8471  from: 1  to: 252



KIEE5124.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AflIII A'CryG_T

Cuts at:      0     164     252 
   Size:        164      88
 
BstBI TT'CG_AA

Cuts at:      0     237     252 
   Size:        237      15
 
BtsI GCAGTG_nn'

Cuts at:      0     165     252 
   Size:        165      87
 
HaeII r_GCGC'y

Cuts at:      0       7      28     252 
   Size:          7      21     224

  Fragments arranged by size: 

                 224      21       7
 
NspI r_CATG'y

Cuts at:      0     168     252 
   Size:        168      84
 
PciI A'CATG_T

Cuts at:      0     164     252 
   Size:        164      88
 
SspI AAT'ATT

Cuts at:      0     225     252 
   Size:        225      27
 
TatI w'GTAC_w

Cuts at:      0     187     252 
   Size:        187      65
 

 Enzymes that do cut:

   AflIII    BstBI     BtsI    HaeII     NspI     PciI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI