(Linear) (Six-base) MAPSORT of: KIEE5123.seq  Check: 6668  from: 1  to: 459



KIEE5123.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     376     459 
   Size:        376      83
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     173     459 
   Size:        173     286
 
AlwNI CAG_nnn'CTG

Cuts at:      0     313     459 
   Size:        313     146
 
BfrBI ATG'CAT

Cuts at:      0     278     459 
   Size:        278     181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     459 
   Size:         47     412
 
BsiHKAI G_wGCw'C

Cuts at:      0      67      98     459 
   Size:         67      31     361

  Fragments arranged by size: 

                 361      67      31
 
BsmI GAATG_Cn'

Cuts at:      0     278     459 
   Size:        278     181
 
Bsp1286I G_dGCh'C

Cuts at:      0      67      98     459 
   Size:         67      31     361

  Fragments arranged by size: 

                 361      67      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     376     459 
   Size:        376      83
 
BsrDI GCAATG_nn'

Cuts at:      0     425     459 
   Size:        425      34
 
BsrGI T'GTAC_A

Cuts at:      0     396     459 
   Size:        396      63
 
BstEII G'GTnAC_C

Cuts at:      0     289     459 
   Size:        289     170
 
BstYI r'GATC_y

Cuts at:      0     330     459 
   Size:        330     129
 
BtsI GCAGTG_nn'

Cuts at:      0      67     459 
   Size:         67     392
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     459 
   Size:         47     412
 
FspI TGC'GCA

Cuts at:      0      76     459 
   Size:         76     383
 
HincII GTy'rAC

Cuts at:      0     131     459 
   Size:        131     328
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     459 
   Size:        156     303
 
MslI CAynn'nnrTG

Cuts at:      0     155     459 
   Size:        155     304
 
NsiI A_TGCA'T

Cuts at:      0     280     459 
   Size:        280     179
 
SfcI C'TryA_G

Cuts at:      0     437     459 
   Size:        437      22
 
SspI AAT'ATT

Cuts at:      0     249     459 
   Size:        249     210
 
StyI C'CwwG_G

Cuts at:      0     161     459 
   Size:        161     298
 
TatI w'GTAC_w

Cuts at:      0     396     459 
   Size:        396      63
 

 Enzymes that do cut:

     AarI     AlfI    AlwNI    BfrBI     BpmI  BsiHKAI     BsmI Bsp1286I
    BspMI    BsrDI    BsrGI   BstEII    BstYI     BtsI  Eco57MI     FspI
   HincII     MmeI     MslI     NsiI     SfcI     SspI     StyI     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI