(Linear) (Six-base) MAPSORT of: KIEE5122.seq  Check: 703  from: 1  to: 339



KIEE5122.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     339 
   Size:         91     248
 
AflIII A'CryG_T

Cuts at:      0      83     339 
   Size:         83     256
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     325     339 
   Size:        325      14
 
AlwNI CAG_nnn'CTG

Cuts at:      0      69     339 
   Size:         69     270
 
AvaI C'yCGr_G

Cuts at:      0     171     339 
   Size:        171     168
 
BanII G_rGCy'C

Cuts at:      0      55     339 
   Size:         55     284
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      74     339 
   Size:         74     265
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      69     339 
   Size:         69     270
 
BsaHI Gr'CG_yC

Cuts at:      0       7     339 
   Size:          7     332
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      37      67     339 
   Size:         37      30     272

  Fragments arranged by size: 

                 272      37      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     339 
   Size:        127     212
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     339 
   Size:        295      44
 
BsiHKAI G_wGCw'C

Cuts at:      0      55     339 
   Size:         55     284
 
Bsp1286I G_dGCh'C

Cuts at:      0      55     339 
   Size:         55     284
 
BspHI T'CATG_A

Cuts at:      0     323     339 
   Size:        323      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      98     339 
   Size:         98     241
 
BssSI C'ACGA_G

Cuts at:      0     139     339 
   Size:        139     200
 
EarI CTCTTCn'nnn_

Cuts at:      0      35     339 
   Size:         35     304
 
EcoICRI GAG'CTC

Cuts at:      0      53     339 
   Size:         53     286
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     339 
   Size:         91     248
 
EcoO109I rG'GnC_Cy

Cuts at:      0     146     339 
   Size:        146     193
 
HaeII r_GCGC'y

Cuts at:      0     118     339 
   Size:        118     221
 
MluI A'CGCG_T

Cuts at:      0      83     339 
   Size:         83     256
 
PpuMI rG'GwC_Cy

Cuts at:      0     146     339 
   Size:        146     193
 
PstI C_TGCA'G

Cuts at:      0     122     339 
   Size:        122     217
 
SacI G_AGCT'C

Cuts at:      0      55     339 
   Size:         55     284
 
ScaI AGT'ACT

Cuts at:      0      46     339 
   Size:         46     293
 
SfcI C'TryA_G

Cuts at:      0     118     339 
   Size:        118     221
 
SmlI C'TyrA_G

Cuts at:      0      48     339 
   Size:         48     291
 
StyI C'CwwG_G

Cuts at:      0     255     339 
   Size:        255      84
 
TatI w'GTAC_w

Cuts at:      0      44     339 
   Size:         44     295
 

 Enzymes that do cut:

     AcuI   AflIII     AlfI    AlwNI     AvaI    BanII     BplI    BpuEI
    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BssSI     EarI  EcoICRI  Eco57MI EcoO109I    HaeII     MluI    PpuMI
     PstI     SacI     ScaI     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI