(Linear) (Six-base) MAPSORT of: KIEE5121.seq  Check: 2443  from: 1  to: 369



KIEE5121.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     325     369 
   Size:         91     234      44

  Fragments arranged by size: 

                 234      91      44
 
AflIII A'CryG_T

Cuts at:      0     262     369 
   Size:        262     107
 
ApoI r'AATT_y

Cuts at:      0     211     369 
   Size:        211     158
 
AvaI C'yCGr_G

Cuts at:      0      17     369 
   Size:         17     352
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     265     313     369 
   Size:        265      48      56

  Fragments arranged by size: 

                 265      56      48
 
BglII A'GATC_T

Cuts at:      0      21     369 
   Size:         21     348
 
BsaAI yAC'GTr

Cuts at:      0     265     369 
   Size:        265     104
 
BsaWI w'CCGG_w

Cuts at:      0     149     369 
   Size:        149     220
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     322     352     369 
   Size:        322      30      17

  Fragments arranged by size: 

                 322      30      17
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     310     369 
   Size:         58     252      59

  Fragments arranged by size: 

                 252      59      58
 
BseYI C'CCAG_C

Cuts at:      0      86     369 
   Size:         86     283
 
BspEI T'CCGG_A

Cuts at:      0     149     369 
   Size:        149     220
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     254     369 
   Size:        254     115
 
BsrBI CCG'CTC

Cuts at:      0     333     369 
   Size:        333      36
 
BsrDI GCAATG_nn'

Cuts at:      0     158     369 
   Size:        158     211
 
BsrFI r'CCGG_y

Cuts at:      0     278     369 
   Size:        278      91
 
BssSI C'ACGA_G

Cuts at:      0     292     369 
   Size:        292      77
 
BstYI r'GATC_y

Cuts at:      0      21     137     369 
   Size:         21     116     232

  Fragments arranged by size: 

                 232     116      21
 
Bsu36I CC'TnA_GG

Cuts at:      0     205     369 
   Size:        205     164
 
EaeI y'GGCC_r

Cuts at:      0       2     369 
   Size:          2     367
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     325     369 
   Size:         91     234      44

  Fragments arranged by size: 

                 234      91      44
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32     369 
   Size:         32     337
 
MscI TGG'CCA

Cuts at:      0       4     369 
   Size:          4     365
 
MspA1I CmG'CkG

Cuts at:      0      60      90     324     345     369 
   Size:         60      30     234      21      24

  Fragments arranged by size: 

                 234      60      30      24      21
 
NaeI GCC'GGC

Cuts at:      0     280     369 
   Size:        280      89
 
NgoMIV G'CCGG_C

Cuts at:      0     278     369 
   Size:        278      91
 
PmlI CAC'GTG

Cuts at:      0     265     369 
   Size:        265     104
 
PvuII CAG'CTG

Cuts at:      0      60      90     369 
   Size:         60      30     279

  Fragments arranged by size: 

                 279      60      30
 
SmlI C'TyrA_G

Cuts at:      0      17     369 
   Size:         17     352
 
TatI w'GTAC_w

Cuts at:      0     230     369 
   Size:        230     139
 
XhoI C'TCGA_G

Cuts at:      0      17     369 
   Size:         17     352
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AvaI     BbsI    BglII    BsaAI    BsaWI
    BsaXI    BseRI    BseYI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI    BstYI   Bsu36I     EaeI  Eco57MI    Hin4I     MscI   MspA1I
     NaeI   NgoMIV     PmlI    PvuII     SmlI     TatI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI