(Linear) (Six-base) MAPSORT of: KIEE5120.seq  Check: 8516  from: 1  to: 309



KIEE5120.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     309 
   Size:        152     157
 
BsaWI w'CCGG_w

Cuts at:      0      41     309 
   Size:         41     268
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     309 
   Size:        307       2
 
BspEI T'CCGG_A

Cuts at:      0      41     309 
   Size:         41     268
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     268     309 
   Size:        268      41
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      90     309 
   Size:         90     219
 
Bsu36I CC'TnA_GG

Cuts at:      0     287     309 
   Size:        287      22
 
DraI TTT'AAA

Cuts at:      0     143     309 
   Size:        143     166
 
EaeI y'GGCC_r

Cuts at:      0     206     309 
   Size:        206     103
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     309 
   Size:        152     157
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     168     283     309 
   Size:        168     115      26

  Fragments arranged by size: 

                 168     115      26
 
HindIII A'AGCT_T

Cuts at:      0     265     309 
   Size:        265      44
 
StyI C'CwwG_G

Cuts at:      0      83     309 
   Size:         83     226
 

 Enzymes that do cut:

     BpmI    BsaWI    BseRI    BspEI   BstAPI    BstXI   Bsu36I     DraI
     EaeI  Eco57MI     FalI  HindIII     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI