(Linear) (Six-base) MAPSORT of: KIEE5116.seq  Check: 9698  from: 1  to: 441



KIEE5116.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     395     441 
   Size:        395      46
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      29     217     441 
   Size:         29     188     224

  Fragments arranged by size: 

                 224     188      29
 
AlwNI CAG_nnn'CTG

Cuts at:      0     399     441 
   Size:        399      42
 
BamHI G'GATC_C

Cuts at:      0     297     441 
   Size:        297     144
 
BbvCI CC'TCA_GC

Cuts at:      0      17     441 
   Size:         17     424
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      78     112     441 
   Size:         78      34     329

  Fragments arranged by size: 

                 329      78      34
 
BlpI GC'TnA_GC

Cuts at:      0     260     441 
   Size:        260     181
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     441 
   Size:         92     349
 
Bpu10I CC'TnA_GC

Cuts at:      0      17     441 
   Size:         17     424
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     441 
   Size:        229     212
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34      85     441 
   Size:         34      51     356

  Fragments arranged by size: 

                 356      51      34
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     400     441 
   Size:        259     141      41

  Fragments arranged by size: 

                 259     141      41
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     155     441 
   Size:        155     286
 
BsrDI GCAATG_nn'

Cuts at:      0     416     441 
   Size:        416      25
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     423     441 
   Size:        423      18
 
BstYI r'GATC_y

Cuts at:      0     297     441 
   Size:        297     144
 
BtgI C'CryG_G

Cuts at:      0     189     441 
   Size:        189     252
 
EarI CTCTTCn'nnn_

Cuts at:      0      59     441 
   Size:         59     382
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     395     441 
   Size:         92     303      46

  Fragments arranged by size: 

                 303      92      46
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     270     441 
   Size:        270     171
 
NcoI C'CATG_G

Cuts at:      0     189     441 
   Size:        189     252
 
NspI r_CATG'y

Cuts at:      0     295     441 
   Size:        295     146
 
SmlI C'TyrA_G

Cuts at:      0     244     441 
   Size:        244     197
 
StyI C'CwwG_G

Cuts at:      0      83     176     189     441 
   Size:         83      93      13     252

  Fragments arranged by size: 

                 252      93      83      13
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    BamHI    BbvCI     BcgI     BlpI     BpmI
   Bpu10I    BpuEI    BseRI     BsgI    BsmBI    BsrDI    BstXI    BstYI
     BtgI     EarI  Eco57MI     FalI     NcoI     NspI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI