(Linear) (Six-base) MAPSORT of: KIEE5115.seq  Check: 3492  from: 1  to: 318



KIEE5115.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      73     318 
   Size:         73     245
 
ApaI G_GGCC'C

Cuts at:      0      87     318 
   Size:         87     231
 
ApoI r'AATT_y

Cuts at:      0     130     318 
   Size:        130     188
 
AvaI C'yCGr_G

Cuts at:      0     212     318 
   Size:        212     106
 
BanI G'GyrC_C

Cuts at:      0     207     318 
   Size:        207     111
 
BanII G_rGCy'C

Cuts at:      0      87     312     318 
   Size:         87     225       6

  Fragments arranged by size: 

                 225      87       6
 
Bme1580I G_kGCm'C

Cuts at:      0      87     318 
   Size:         87     231
 
BsiHKAI G_wGCw'C

Cuts at:      0     312     318 
   Size:        312       6
 
Bsp1286I G_dGCh'C

Cuts at:      0      87     312     318 
   Size:         87     225       6

  Fragments arranged by size: 

                 225      87       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73     318 
   Size:         73     245
 
BstYI r'GATC_y

Cuts at:      0      92     318 
   Size:         92     226
 
BtsI GCAGTG_nn'

Cuts at:      0     255     318 
   Size:        255      63
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     257     318 
   Size:        257      61
 
EcoICRI GAG'CTC

Cuts at:      0     310     318 
   Size:        310       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0      83     318 
   Size:         83     235
 
EcoRV GAT'ATC

Cuts at:      0     141     318 
   Size:        141     177
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      84     116     244     276     294     318 
   Size:         84      32     128      32      18      24

  Fragments arranged by size: 

                 128      84      32      32      24      18
 
MfeI C'AATT_G

Cuts at:      0     103     318 
   Size:        103     215
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     195     227     318 
   Size:        195      32      91

  Fragments arranged by size: 

                 195      91      32
 
PspOMI G'GGCC_C

Cuts at:      0      83     318 
   Size:         83     235
 
PspXI vC'TCGA_Gb

Cuts at:      0     212     318 
   Size:        212     106
 
PstI C_TGCA'G

Cuts at:      0      79     317     318 
   Size:         79     238       1

  Fragments arranged by size: 

                 238      79       1
 
SacI G_AGCT'C

Cuts at:      0     312     318 
   Size:        312       6
 
SfcI C'TryA_G

Cuts at:      0      75     313     318 
   Size:         75     238       5

  Fragments arranged by size: 

                 238      75       5
 
SmlI C'TyrA_G

Cuts at:      0       5     212     318 
   Size:          5     207     106

  Fragments arranged by size: 

                 207     106       5
 
XhoI C'TCGA_G

Cuts at:      0     212     318 
   Size:        212     106
 

 Enzymes that do cut:

     AarI     ApaI     ApoI     AvaI     BanI    BanII Bme1580I  BsiHKAI
 Bsp1286I    BspMI    BstYI     BtsI     EciI  EcoICRI EcoO109I    EcoRV
    Hin4I     MfeI     PpiI   PspOMI    PspXI     PstI     SacI     SfcI
     SmlI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  Eco57MI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI