(Linear) (Six-base) MAPSORT of: KIEE5112.seq  Check: 7851  from: 1  to: 432



KIEE5112.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AccI GT'mk_AC

Cuts at:      0     240     432 
   Size:        240     192
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     432 
   Size:        328     104
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     422     432 
   Size:        422      10
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     389     432 
   Size:        389      43
 
Bpu10I CC'TnA_GC

Cuts at:      0     107     432 
   Size:        107     325
 
DraI TTT'AAA

Cuts at:      0      84     278     432 
   Size:         84     194     154

  Fragments arranged by size: 

                 194     154      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     389     432 
   Size:        328      61      43

  Fragments arranged by size: 

                 328      61      43
 
EcoRV GAT'ATC

Cuts at:      0     429     432 
   Size:        429       3
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      43      75     432 
   Size:         43      32     357

  Fragments arranged by size: 

                 357      43      32
 
NdeI CA'TA_TG

Cuts at:      0     315     432 
   Size:        315     117
 
NspI r_CATG'y

Cuts at:      0     337     432 
   Size:        337      95
 
PfoI T'CCnGG_A

Cuts at:      0     366     432 
   Size:        366      66
 
TatI w'GTAC_w

Cuts at:      0     281     432 
   Size:        281     151
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     133     165     348     380     432 
   Size:        133      32     183      32      52

  Fragments arranged by size: 

                 183     133      52      32      32
 

 Enzymes that do cut:

     AccI     AcuI     BbsI     BpmI   Bpu10I     DraI  Eco57MI    EcoRV
    Hin4I     NdeI     NspI     PfoI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI